沙眼衣原体感染HeLa229细胞Bim表达及抑制凋亡研究  

Expression of Bim in Chlamydia trachomatis-Infected HeLa229 Cells and Apoptosis Inhibition

作　　者：杨志英[1] 欧录明[1] 陈福春[1] 胡永轩[1] 余平[2] 

机构地区：[1]湘南学院基础医学课部,湖南郴州423000 [2]中南大学免疫学系,湖南长沙410078

出　　处：《微生物学杂志》2008年第2期44-48,共5页Journal of Microbiology

基　　金：湖南省教育厅基金项目(06C787)

摘　　要：研究沙眼衣原体(D血清型)感染的HeLa229细胞中Bim蛋白质的表达及凋亡诱导剂作用后的凋亡情况。Western-blot检测沙眼衣原体感染和未感染的HeLa229细胞Bim蛋白质的表达水平。凋亡诱导剂etopo- side作用HeLa229细胞后,经Hoechst33258染色用荧光显微镜观察核浓缩和凋亡小体;流式细胞仪检测凋亡率。HeLa229细胞在未感染及感染沙眼衣原体6 h后可检测到Bim的表达;在感染24、48 h后均未检测到Bim的表达。经etoposide作用后,未感染的HeLa229细胞观察到明显的核浓缩和凋亡小体;流式细胞仪检测的凋亡率为90.64%。感染24 h的HeLa229细胞,未观察到核浓缩和凋亡小体;流式细胞仪检测的凋亡率为11.50%,与未感染的HeLa229细胞诱导后的凋亡率比较有统计学意义(P<0.05)。沙眼衣原体感染HeLa229细胞后可降低Bim蛋白质的表达;并能抑制etoposide诱导的细胞凋亡。Expression of Bim in Chlamydia trachomatis （Ct） （Serotype D）-infected HeLa229 cells and their apoptosis after the effect of etoposide induction were studied. Western-blot was adopted to detect the expression of Bim at protein level of Ct-infected and Ct-uninfected HeLa229 cells. And after the effect of etoposide on HeLa229 cells, nuclear condensation and apoptotic corpus were observed with fluorescence microscope after Hoechst 33258 staining, and apoptotlc rate was examined with flow cytometer （FCM） assay. The results showed that the expression of Bim was de- tected both in Ct-infected and Ct-uninfected HeLa229 cells 6 hours after the infection, however, 24 hours and 48 hours after the infection, no expression of Bim was detected. After etoposide induction, obvious nuclear condensation and apoptotic corpus were observed in HeLa229 cells, the apoptotic rate with FCM was 90.64%. However, 24 hours after the infection, no nuclear condensation and apoptotic corpus were observed, and the apoptotic rate with FCM was 11.50%. As compared with apoptotic rate of uninfected HeLa229 cells after the induction there was statistic significance （P 〈0.05）. Therefore, the expression of Bim after Ct infection could be reduced, and could inhibit apoptosis induced by etoposide.

关 键 词：沙眼衣原体(D血清型) HELA229细胞 BIM 细胞凋亡 

分 类 号：R374[医药卫生—病原生物学]