小鼠血管平滑肌细胞原代培养方法的改良  

IMPROVEMENT OF THE METHODS FOR PRIMARY CULTURE OF MOUSE VASCULAR SMOOTH MUSCLE CELL

作　　者：吴建芳[1] 

机构地区：[1]青海大学医学院诊断教研室

出　　处：《青海医学院学报》2008年第1期52-53,共2页Journal of Qinghai Medical College

摘　　要：目的建立一种简单方便但高效的血管平滑肌细胞原代培养方法。方法改良酶消化法。结果用该法培养细胞传代生长快,细胞纯度达98%以上,足以满足各种细胞试验需求。结论与传统方法相比该法简单经济,试验成功率高,节省材料和时间,而且可获得更多纯度较高的细胞。Objective To create a simple and effective primary culture methods for mouse vascular smooth muscle cells（VSMC）.Methods Reformed enzymatic digestion.Results VSMC was grown well and fast,the purity is more than 98% which can meet various cell tests.Conclusion Comparing with traditional methods,this approaches can save much time and more materials,also have better cell quantities and purity.

关 键 词：小鼠 动脉血管平滑肌细胞 原代培养 

分 类 号：R329.27[医药卫生—人体解剖和组织胚胎学]