益气养阴、活血化瘀方对实验性病毒性扩张型心肌病小鼠外周血γ-干扰素的影响  

Effect of the Prescription of Yiqiyangyin Huoxuehuayu on the Level of Serum γ-IFN in Mice Model with Viral Dilated Cardiomyopathy

作　　者：赵艳霞[1] 周亚滨[1] 崔志勇[1] 张晶[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中国中医药信息杂志》2008年第6期25-27,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：黑龙江省杰出青年基金(JC0208)

摘　　要：目的通过检测小鼠外周血γ-干扰素(γ-IFN)水平,探讨益气养阴、活血化瘀方对病毒性扩张型心肌病小鼠的作用机制。方法Balb/c小鼠210只,随机分为4组,空白组30只,模型组及中药高、低剂量组各60只。采用多次腹腔注射柯萨奇B3m病毒的方式,建立重复感染的早期病毒性扩张型心肌病动物模型。中药高、低剂量组用益气养阴、活血化瘀方治疗,于治疗4周后观测小鼠心肌病理形态学改变、死亡率、体重、心脏质量指数变化,双抗体夹心ELISA法测定外周血γ-IFN水平。结果益气养阴、活血化瘀方能促进病毒性扩张型心肌病小鼠γ-IFN诱生,中药高、低剂量组与模型组实验结果经统计学处理有显著差异。结论益气养阴、活血化瘀方具有抗病毒、调节免疫功能的作用。Objective To investigate the effect of the prescription of Yiqiyangyin Huoxuehuayu on the mice model with viral dilated cardiomyopathy by examining the levels of serum γ-IFN. Methods Replicated the animal models of early viral dilated cardiomyopathy by injecting the CVB3m virus to the peritoneal cavity of the Balb/c mice. Two hundred and ten Balb/c mice were divided into 4 groups at random： blank group, model group, high and low dose traditional Chinese medicine group. The blank group has 30 mice. The other three group has 60 mice each. The mice of high dose traditional Chinese medicine group and low dose traditional Chinese medicine group were treated with the prescription of Yiqiyangyin Huoxuehuayu. The change of pathomorphology in the cardiac muscle tissue, the rate of death, the weight change and the cardiac weight index were observed after 4 weeks of the treatment. At the same time, ELISA method was used to exam the levels of serum γ-IFN. Results The prescription of Yiqiyangyin Huoxuehuayu can induce the expression of serum γ-IFN in the mice with viral dilated cardiomyopathy. The mean level of serum γ-IFN of the high dose traditional Chinese medicine group and low dose traditional Chinese medicine group were significanfly higher than that of the model group. Conclusions The prescription of Yiqiyangyin Huoxuehuayu can inhibit the viral replication and regulate the immunologic function.

关 键 词：益气养阴 活血化瘀方 Γ-干扰素 病毒性扩张型心肌病 柯萨奇B3M病毒 

分 类 号：R289.11[医药卫生—方剂学]