尿中碘化物气相色谱法测定  

Determination of iodide in urine by gas chromatography

作　　者：赵晶[1] 杨楠[1] 张欣[1] 赵雪红[1] 

机构地区：[1]沈阳市疾病预防控制中心,沈阳110031

出　　处：《中国卫生检验杂志》2008年第5期813-814,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立尿中碘化物的气相色谱测定方法。方法：采用重铬酸钾、硫酸、丁酮与碘酸钾反应,用苯作萃取剂,HP-5毛细管柱分离,ECD检测器检测,峰面积定量。结果：本法检出限为0.025μg/ml,I^-在0-1.0μg/ml浓度范围内呈线性关系,平均回收率为82.7%-93.2%,相对标准偏差（RSD）为3.0%,与砷铈法比较P〉0.05,无显著性差异,尿中氟化物、氯化物不干扰碘化物的测定。结论：该法操作简便、灵敏、准确可靠,用于实际尿样的测定,具有较高应用价值。Objective：To develop a gas chromatography method for the determination of iodide in urine.Methods：Potassium iodate in urine samples was reacted with potassium dichromate,sulfuric acid,2-butanone and extracted by benzene.The analyte was separated on HP-5 capillary column and detected by electron capture detector（ECD）,quantified by peak areas.Results：The limit of quantification was 0.025 μg/ml.The calibration curve showed good linearity in the range of 0-1.0 μg/ml.The mean recovery was between 82.7% and 93.2% and the relative standard derivation 3.0%.Fluoride and choloride in urine samples didn′t effected on the determination of iodide.It had no significant difference with As-Ce catalysis spectrophotometry（P〉0.05）.Conclusion：This method is simple,sensitive and accurate.It is reliable to determine the iodide in urine.

关 键 词：毛细管气相色谱 衍生 尿碘 

分 类 号：O657.71[理学—分析化学]