胶束电动毛细管电泳法分离6种生物碱基  

Separation of six biological bases by micellar electrokinetic capillary chromatography

作　　者：徐芳[1] 陈波[1] 姚守拙[1] 

机构地区：[1]化学生物学及中药分析省部共建教育部重点实验室 湖南师范大学,长沙410081

出　　处：《药物分析杂志》2008年第5期678-681,共4页Chinese Journal of Pharmaceutical Analysis

基　　金："973""中药药性成因研究"课题;编号:2006CB504701

摘　　要：目的:采用胶束电动毛细管电泳法(MECC)分离腺嘌呤(A)、鸟嘌呤(G)、胞嘧啶(C)、胸腺嘧啶(T)、茶碱和咖啡因6种生物碱基。方法:20℃、18 kV 应用电压下,检测波长270 nm,在 35 mmol·L^(-1)十二烷基硫酸钠(SDS)-10 mmol·L^(-1)硼砂(pH 9.0)运行缓冲液中电泳分离。结果:6种碱基8 min 内达到基线分离;在10～500 mg·L^(-1)浓度范围内呈良好线性关系;相关系数为0.9995～0.9999;检出限为12.5～100μg·L^(-1),加样回收率在96.1%～105.2%。并用于3种药物和茶叶中生物碱基含量的测定。结论:方法简便、快速,灵敏度高,可用于分析和测定含生物碱基的药物和饮料。Objective：To investigate the application of micellar electrokinetic capillary chromatography for separation and determination six biological bases [cytosine（C） , thymine（T） , adenine（A） , caffeine, theophylline and guanine（G） ]. Method：At 20 ℃ and 18 kV applied voltage, on - line UV detector was operated at 270 nm, six biological bases introduced into the capillary separation reached the basesline separation in 8 minutes in 35 mmol · L^-1 SDS- 10 mmol · L^-1 sodium tetraborate buffer solution（ adjusted to pH 9.0 with hydrochloric acid）. Results： The concentration ranges of the linearity were all about 10 -500 mg · L^-1 ,and the correlation coefficient was 0. 9995 - 0. 9999 ,the limit of detection（S/N =3 ）was 12.5 - 100 μg · L^-1 ,the recovery was 96. 1% - 105.2%. This method was adopted for the determination of biological bases in Qutong tablets, ganciclovir for injection, Xinganbao capsules and tea. Conclusion：The method is simple, rapid and accurate.

关 键 词：MECC 腺嘌呤 鸟嘌呤 胞嘧啶 胸腺嘧啶 茶碱 咖啡因 

分 类 号：R917[医药卫生—药物分析学]