HPLC-RI法测定银杏叶提取物中4种内酯的含量  

HPLC-RI determination of four ginkgolides in Ginkgo biloba extracts

作　　者：袁传勋[1] 潘见[1] 胡学桥[1] 开桂青[1] 

机构地区：[1]合肥工业大学农产品生物化工教育部工程研究中心,合肥230069

出　　处：《药物分析杂志》2008年第5期739-741,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金重点项目(20136020);教育部科技创新工程重大项目培育基金项目(7074027)

摘　　要：目的:建立高效液相色谱法测定银杏叶提取物中4种内酯(银杏内酯 A、银杏内酯 B、银杏内酯 C、白果内酯)的含量。方法:采用 Symmetryshield 型 C_(18)色谱柱(3.9 mm×150 mm,5 μm),以甲醇-水(30:70)为流动相,流速为1.0 mL·min~^(-1),用外标法测定,示差折光检测器检测。结果:4种内酯浓度在0.02～0.24 mg·mL^(-1)范围内,与色谱峰面积呈良好线性关系,相关系数在0.9984～0.9998之间,检测限在0.025～0.035 μg之间,回收率在99.0%～100.2%之间。结论:本方法简单、准确、可靠、灵敏,适用于实验室和企业常规质量控制。Objective：To establish an HPLC method for the determination of four ginkgolides [ ginkgolide A （ GA）, ginkgolide B （ GB）, ginkgolide C （ GC ）, and bilobalide （ BB ） ] in Ginkgo biloba extracts （GBE）. Methods ： The Symmetryshield CI8 （3.9 mm × 150 mm,5 μm）column was used,the mobile phase consisted of methanol- water（30： 70） at a flow rate of 1.0 mL · min^- 1, the detection was carried out with refractive index detector. Four ginkgolides were measured with the external standard method. Results ： The linear ranges of four ginkgolides were 0.02 - 0. 24 mg· mL^-1 （correlation coefficients were between 0. 9984 - 0. 9998） ; The limits were between 0. 025 - 0. 035 μg ; Recoveries were 99.0% -100.2% ,respectively. Conclusion：The method is simple,accurate and sensitive. It is suitable for the routine quality control.

关 键 词：银杏内酯A 银杏内酯B 银杏内酯C 白果内酯 示差折光检测器 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]