反相高效液相色谱法测定胃炎片中芍药苷的含量  

RP-HPLC determination of paeoniflorin in Weiyan tablets

作　　者：赵庆华[1] 王海龙[1] 王宝全[1] 

机构地区：[1]济南军区联勤部药品仪器检验所,济南250022

出　　处：《药物分析杂志》2008年第5期796-797,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立胃炎片中芍药苷的含量测定方法。方法:采用反相高效液相色谱法测定胃炎片中芍药苷的含量。使用日本Sil C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸水溶液(13:87)为流动相,流速为1 mL·min^(-1),检测波长230nm。结果:芍药苷进样量存0.14～1.40μg范围内线性关系良好,r=0.9994;平均回收率(n=9)为101.3%。结论:该方法简便、灵敏、准确,可作为胃炎片的质量控制方法。Objective：To develop an HPLC method for the determination of paeoniflorin in Weiyan tablets. Methods：The analysis was carried out on Japanese Sil C18 column（250 mm ×4.6 mm,5 μm） ,with acetonitrile -0. 1% phosphoric acid （ 13 ： 87 ） as mobile phase （ 1 mL · min ^- 1 ） and detecting wavelength at 230 nm. Results： The linear range of paeoniflorin was in 0. 14 - 1.40μg and the correlation coefficient was 0. 9994. The average recovery rate （n = 9）was 101.3%. Conclusion：The method is convenient and accurate,and can be used for quality control.

关 键 词：高效液相色谱 胃炎片 芍药苷 

分 类 号：R917[医药卫生—药物分析学]