RP-HPLC测定注射用复方甘草酸苷中甘草酸铵含量和有关物质  

RP-HPLC determination of ammonium glycyrrhizinate and related substances in compound glycyrrhizin for injection

作　　者：张敏娟[1] 

机构地区：[1]青海省药品检验所,西宁810000

出　　处：《药物分析杂志》2008年第5期798-800,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用高效液相色谱法测定注射用复方甘草酸苷中甘草酸铵的含量和有关物质。方法:采用 Symmetry C_(18)色谱柱(4.6 mm×250 mm,5 μm),以2%醋酸-乙腈(65:35)为流动相,流速为1.0 mL·min^(-1),检测波长为256 nm,进样量为20μL。结果:甘草酸铵线性范围为40～400μg·mL^(-1)(r=0.9999),最小检测量为6.23 ng。结论:本法精密度好,结果准确、可靠、灵敏,可用于注射用复方甘草酸苷含量测定和有关物质检查。Objective：To develop an HPLC method for the determination of ammonium glycyrrhizinate and related substances in compound glycyrrhizin for injection. Methods： The chromatography was performed on Symmetry C18 column（4. 6 mm × 250 mm,5 μm）with 2% acetic acid- acetonitrile（65：35 ）as the mobile phase at a flow rate of 1.0 mL · min^-1and the absorbance was monitored at 256 nm. The injection volume was 20μL. Results：The calibration curves of ammonium glycyrrhizinate was linear in the range of 40 - 400 μg · mL^- 1 （ r = 0. 9999 ）. The limit of detection was 6. 23 ng. Conclusion ： The method is accurate, credible and sensitive. It can be used for controlling the quality of compound glycyrrhizin for injection.

关 键 词：高效液相色谱法 注射用复方甘草酸苷 甘草酸铵 有关物质 

分 类 号：R917[医药卫生—药物分析学]