云南景洪直立型普通野生稻抗稻瘟病Pi-ta^＋等位基因的克隆与分析  

Cloning and analyzing of rice blast resistance gene Pi-ta^+ allele from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan

作　　者：耿显胜[1] 杨明挚[1] 黄兴奇[2] 程在全[2] 付坚[2] 孙涛 李俊 

机构地区：[1]云南大学生命科学学院,昆明650091 [2]云南省农业科学院生物技术与种质资源研究所,昆明650223 [3]云南省西双版纳州农业科学研究所,景洪666100

出　　处：《遗传》2008年第1期109-114,共6页Hereditas（Beijing)

基　　金：云南省自然科学基金重点项目(编号:2004C0010Z);云南省自然科学基金(编号:2006C00007Q)~~

摘　　要：用PCR法从景洪直立紫杆普通野生稻中克隆了抗稻瘟病基因Pi-ta+的4672bp序列,该序列包含完整的编码框、内含子和终止密码子下游的331bp。所克隆的直立型紫杆普通野生稻Pi-ta基因序列的编码区与已报道的日本栽培稻社糯(Yashiro-mochi)和元江普通野生稻相应序列间的同源性分别为99.86%和98.78%。与社糯的Pi-ta基因相比,其编码区有4个核苷酸的差异并导致3个氨基酸残基的改变,而内含子区域有6个核苷酸差异。对该序列进一步分析发现,其推导的氨基酸残基的918位为丙氨酸,属于稀有的抗稻瘟病的Pi-ta+等位基因。景洪直立型普通野生稻Pi-ta+基因因其编码序列和推导的氨基酸序列与社糯有所不同,推测其抗病能力大小和抗菌谱可能与社糯的Pi-ta基因不同。直立型普通野生稻中Pi-ta+等位基因的克隆为进一步利用该基因改良栽培稻抗病能力提供了前期物质基础。A 4 672 bp DNA sequence including the whole coding region and partial non-coding region of rice blast resistance gene Pi-ta^＋ has been cloned from Jinghong erect type of common wild rice （Oryza rufipogon Griff） in Yunnan by polymerase chain reaction method. The coding region shares 99.86% and 98.78% identity with the corresponding regions of the reported cultivated rice Yashiro-mochi and Yuanjiang type of common wild rice respectively. There are 4 nucleotides difference in the coding region and 6 in intron of the cloned Pi-ta^＋ gene,compared with Pi-ta from Yashiro-mochi. Pi-ta^＋ gene in Jinghong erect type of common wild rice has been proved to be a rare existing Pi-ta^＋ allele, because there was a alanine rather than a serine at the position 918 within the predicted amino acid sequence of PITA. Pi-ta^＋ allele can cause disease resistance response to rice blast pathogens in plant cells. Differences in DNA sequence, deduced amino acid se- quence and antibacterial spectrum may make the Pi-ta^＋ allele new resistant characteristics. Finding and cloning of Pi-ta^＋ allele from Jinghong erect type of common wild rice in Yunnan provides a basement for further utilization of the wild rice resources.

关 键 词：景洪直立型普通野生稻 Pi-ta+等位基因 基因克隆 DNA多态性 

分 类 号：S511.9[农业科学—作物学]