微卫星座位与丝羽乌骨鸡蛋用系开产性状相关分析  

Correlation analysis between microsatellite loci and traits at first laying in egg-laying lines of silkies

作　　者：康丽[1] 朱庆[1] 

机构地区：[1]四川农业大学动物科技学院

出　　处：《遗传》2008年第2期203-208,共6页Hereditas（Beijing)

基　　金：四川省畜禽育种攻关项目(编号:01NG002-06)~~

摘　　要：采用多重PCR扩增结合荧光标记全自动电泳检测技术,对鸡基因组1号染色体和Z染色体上20个微卫星座位与丝羽乌骨鸡BM、BF两个蛋用新品系180个个体的开产性状进行相关性分析。结果表明:位于1号染色体上的MCW0068、MCW0200与开产蛋重相关显著(P<0.05);位于1号染色体上的MCW0068、MCW0208和位于Z染色体上的MCW0128与开产体重相关显著(P<0.05);而位于Z染色体上的MCW0154与开产体重相关极显著(P<0.01)。Twenty microsateUite loci on Chromosome 1 and Chromosome Z were selected from chicken genome for correlation analysis with traits at first laying in silkies. The 180 silkies were from two egg-laying lines of BM and BE Genotyping of microsatellite loci was carried out by using fluorescein-labeled multiplex-PCR detection technology. The results showed that MCWO068 and MCW0200 on Chromosome 1 were significantly associated with egg weight at first laying （P〈0.05）, MCWO068 and MCW0208 on Chromosome 1 and MCWO128 on Chromosome Z were significantly associated with body weight at first laying （P〈0.05）, and MCWO154 on Chromosome Z was highly significantly associated with body weight at first laying （P〈0.01）.

关 键 词：丝羽乌骨鸡 微卫星标记 多重PCR 开产性状 

分 类 号：S831[农业科学—畜牧学]