水稻ILP标记遗传图谱的构建  

Construction of a genetic map based on ILP markers in rice

作　　者：赵向前[1] 吴为人[1] 

机构地区：[1]浙江大学农业与生物技术学院

出　　处：《遗传》2008年第2期225-230,共6页Hereditas（Beijing)

基　　金：国家高新技术研究发展计划项目(863技术)(编号:2006AA10Z1E2)~~

摘　　要：内含子长度多态性(ILP)是一种基于PCR的新型分子标记,具有许多突出的优点。我们先前利用已公布的籼稻品种93-11和粳稻品种日本晴的基因组序列数据,已开发了172个水稻ILP标记。为了检验这些ILP标记的可靠性及其在遗传作图中的可用性,利用一个BC1F1(日本晴/93-11//日本晴)群体,构建了一张含172个ILP标记座位和13个SSR标记座位的水稻遗传图谱,总长度为1905.7cM。比较显示,图谱上所有标记的顺序与其物理顺序完全一致,证明了利用ILP标记进行遗传作图的可行性和有效性。文中还对标记偏分离现象进行了分析,发现在第6号染色体短臂上存在一个严重偏分离的区域。Intron length polymorphism （ILP） is a new type of PCR-based molecular markers with many advantages. We had previously developed 172 ILP markers in rice using the published genome sequence data of indica cultivar 93-11 and japonica cultivar Nipponbare. In order to examine the reliability and the applicability of these ILP markers to genetic mapping, we constructed a rice genetic map consisting of 172 ILP and 13 SSR markers with a total length of 1 905.7 cM using a BC1F1 （Nipponbare/93-11//Nipponbare） population. Comparison showed that the order of the markers in this map was exactly the same as their physical order, verifying the feasibility and efficiency of using ILP markers for genetic mapping. We also investigated the phenomenon of marker segregation distortion and found a region of serious segregation distortion in the short arm of chromosome 6.

关 键 词：水稻 ILP标记 遗传图谱 

分 类 号：S511[农业科学—作物学]