小麦细胞核仁中DNA原位位置与rRNA基因转录位点的研究  

Localization of nucleolar DNA and transcription sites of rRNA genes in situ in wheat cells

作　　者：赫杰[1] 陶伟[2] 郝水[3] 

机构地区：[1]哈尔滨工业大学生命科学与工程系,哈尔滨150001 [2]北京大学生命科学学院,北京100871 [3]东北师范大学遗传与细胞研究所,长春130024

出　　处：《遗传》2008年第2期231-236,共6页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:39770367)资助~~

摘　　要：以小麦细胞为研究材料,应用常规电子显微镜技术和DNA细胞化学特异染色NAMA-Ur技术,在原位水平对核仁中DNA的分布和特征进行了直观的观察。结果表明,小麦细胞核仁中DNA位于纤维中心(Fibrillar Centers,FC)、致密纤维组分(Dense Fibrillar Component,DFC)以及两者的过渡区域,并呈现出环绕FC排布的构型;应用RNP优先染色(Benhard staining)技术分析了核仁中RNP的分布及其原位位置,直观的显示了小麦细胞核仁中RNP颗粒主要集中在FC与DFC的过渡区域及DFC和颗粒组分(Granular Component,GC)中;并且在FC与DFC的过渡区域,它们不太均匀也不太连续地半围绕着FC而排布;进一步借助于RNA/DNA杂合体抗体在原位水平标记和分析了细胞核仁中活跃基因转录的精细位点,结果表明小麦细胞核仁rRNA基因的转录位点位于FC与DFC的过渡区域及DFC中。By using the conventional electron microscopic technique and DNA specific cytochemical staining method （NAMA-Ur）, we directly observed the arrangement and location of intranucleolar DNA in situ in wheat （Triticum aestivum L.） cells. The results showed that nucleolar DNA was found in Fibrillar Centers （FC）, Dense Fibrillar Component （DFC） and the transitional region between FC and DFC. Moreover, the nucleolar DNA was distributed along the periphery of FC and by surrounding FC. We employed RNP preference staining （Bernhard staining） method to visualize the distribution and position of RNP in situ in nucleoli of wheat cells. The results directly showed that RNP mainly located in the transitional region between FC and DFC, in DFC and in Granular Component （GC）. Moreover, RNP was irregularly distributed around FC. By employing anti-RNA/DNA hybrid antibodies, we directly and selectively labeled transcription sites of rRNA genes and testified that localization of transcription sites was not only in the transitional region between DFC and FC but also in DFC of nucleoli in wheat cells.

关 键 词：DNA特异染色 RNP优先染色 RNA/DNA杂合体 小麦 

分 类 号：Q943[生物学—植物学] S512.1[农业科学—作物学]