过量表达棉花液泡H^+-ATPase C亚基基因促进酵母细胞伸长和提高盐胁迫耐受性  

Overexpression of cotton, vacuolar H^+-ATPase subunit C promotes cell elongation and regulates stress tolerance in fission yeast

作　　者：肖忠意[1] 谭琨岭[1] 胡明瑜[1] 罗明[1] 

机构地区：[1]西南大学生物技术中心,重庆400716

出　　处：《遗传》2008年第4期495-500,共6页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:30370904和30671258);国家高技术研究发展计划(863计划)(编号:2006AA10Z121)资助~~

摘　　要：棉花液泡H+-ATPase在纤维细胞的伸长过程中具有重要作用,能够通过对纤维细胞膨压的调节而介导细胞的极性膨大。拟南芥液泡H+-ATPase C亚基(DET3)具有调节液泡H+-ATPase活性,进而调控细胞伸长的作用。为了快速鉴定棉花液泡H+-ATPaseC亚基基因(GhDET3)的功能和推测其在棉花纤维生长中的作用,作者构建了GhDET3的酵母表达载体pREP5N(+)-GhDET3,并进行了裂殖酵母的遗传转化。结果表明,在酵母细胞中过量表达GhDET3基因,能够促进酵母细胞的伸长和提高酵母细胞对NaCl和高pH的耐受性,说明GhDET3对液泡H+-ATPase的活性有重要影响,由此推测GhDET3基因与棉花纤维细胞的伸长生长具有密切关系。Cotton （Gossypium hirsuturm L.） vacuolar H^＋-ATPase plays an important role in fiber cell elongation through regulating the turgor pressure, which is involved in polarity enlargement of fiber cell. Arabidopsis vacuolar H^＋-ATPase subunit C （DET3） regulates the activity of vacuolar H^＋-ATPase and subsequently promotes cell elongation. To rapidly characterize the function of cotton vacuolar H^＋-ATPase subunit C （GhDET3） and speculate the role of GhDET3 in cotton fiber growth and development, we constructed an expressing vector for fission yeast and intxoduced it into fission yeast. The results revealed that overexpression of GhDET3 was capable of promoting fission yeast cell elongation and enhancing the tolerance to high NaCI and high pH stresses. These results illuminated that the effects of GhDET3 were important for the activity of vacuolar H^＋-ATPase and suggested that GhDET3 plays a crucial role in fiber cell elongation.

关 键 词：棉花 液泡H^+-ATPase C亚基 裂殖酵母 GhDET3 

分 类 号：S562[农业科学—作物学] Q943[生物学—植物学]