感觉神经肽P物质在糖尿病大鼠皮肤损伤修复中的应用  

Application of exogenous substance P in wound healing of skin injury in diabetic rat

作　　者：郭文治[1] 赖西南 王正国[1] 刘育杰[1] 王丽丽[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤烧伤与复合伤重点实验室,重庆400042

出　　处：《创伤外科杂志》2008年第3期251-253,共3页Journal of Traumatic Surgery

基　　金：国家重点基础研究发展计划(G1999054200)

摘　　要：目的观察外源性感觉神经肽P物质(SP)在糖尿病大鼠皮肤伤口愈合中的作用。方法链脲佐菌素(STZ)致糖尿病大鼠48只,行鼠背部脊柱两侧皮肤切割伤口后,随机分为实验组与对照组,每组24只。实验组伤后在伤口四周注射10-7mol/L的SP,每日2次,每个伤口200μl/次,连续注射4天;对照组注射等量SP溶剂生理盐水。伤后4、7、10、14、17、21天用透明膜描记法记录伤口愈合面积,伤后4、7、10天取材检测肉芽组织羟脯氨酸总量和Ⅰ型、Ⅲ型胶原含量。结果实验组在伤后4、7、10、14天伤口愈合面积明显高于对照组(P<0.01),愈合时间比对照组缩短1周;伤后4、7、10天肉芽组织中羟脯氨酸含量、Ⅰ型、Ⅲ型胶原含量明显高于对照组(P<0.01)。结论应用外源性SP明显促进糖尿病大鼠皮肤创伤愈合。Objective To study the effects of substance P（SP） on improving the wound healing of skin injury in diabetic rat.Methods Forty-eight diabetic rats,each rat had two round-shaped-wounds through the whole skin by the side of the spine on the back.The rats were divided into two groups： control group and experimental group,each group consisted of 24 rats.200μl of substance P,10^-7each wound,was injected around each wound of the experimental group,two times a day.While the same volume of normal saline was injected in the same site to the control group.The area of the wounds was measured at different time.The content of hydroxyproline was measured at the same time.The changes of the collagen type I and collagen Ⅲ were estimated by immunohistochemical staining.The healing time was recorded after wounding.Results The wound area decreased obviously in the experimental group as compared with control group on the 4th,7th,10th,14th day after injury（P〈0.01）.The healing time shortened in the experimental group as compared with the control group.The content of hydroxyproline within granulation tissue increased on the 4th,7th,10th day.The same changes with the collagen type Ⅰ and collagen Ⅲ（P〈0.01） were found.Conclusion The healing of the wound on the back of diabetic rat was improved by substance P.

关 键 词：感觉神经肽P物质 糖尿病 皮肤 伤口愈合 

分 类 号：R392.1[医药卫生—免疫学] R454.2[医药卫生—基础医学]