尾型同源盒基因2基因表达对胃癌细胞生长影响的研究  

Caudal-related homeobox 2 gene expression and its effects on human gastric carcinoma cell line

作　　者：李雷[1] 肖强[1] 谢玉波[2] 马玉林[1] 唐振勇[1] 尹永硕[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,南宁530021 [2]广西医科大学第一附属医院麻醉科,南宁530021

出　　处：《中华外科杂志》2008年第11期847-850,共4页Chinese Journal of Surgery

基　　金：广西科学基金资助项目（桂科回0448013）

摘　　要：目的观察尾型同源盒基因2（Cdx2）基因过表达对胃癌细胞生物学性状的影响。方法构建人Cdx2真核表达载体，并转染胃癌细胞MGC-803。实验分为四组：未转染组、转染pCMV-HA组、转染pCMV-GAPDH-HA组、转染pCMV-Cdx2-HA组。应用荧光定量PCR检测各组Cdx2基因的表达情况，Western blot法检测各组Cdx2蛋白的表达情况，MTT法观测细胞增殖，透射电镜观察转染细胞形态学的改变，流式细胞仪分析细胞周期和凋亡。结果成功构建了pCMV-Cdx2-HA真核表达载体，在瞬时转染pCMV-Cdx2-HA质粒48h的MGC-803细胞有较高水平的Cdx2基因mRNA和蛋白的表达；与未转染组、转染pCMV-HA组、转染pCMV-GAPDH-HA组比较，转染pCMV-Cdx2-HA组的胃癌细胞在转染72h时生长受到明显抑制，G0／C1期比例上升，S期比例下降，胃癌细胞的凋亡率也明显升高（P〈0．05）；转染pCMV-Cdx2-HA的胃癌细胞胞核消失，核染色质固缩、解体，内质网、高尔基复合体膨大成泡状。结论Cdx2基因可明显抑制胃癌细胞的生长，提示Cdx2参与了胃癌的生长调控。Objective To investigate the effect of human caudal-related homeobox 2 （Cdx2） gene expression on human gastric carcinoma cell line MGC-803. Methods pCMV-Cdx2-HA eukaryotic expression plasmid was constructed by using DNA recombinant method. The MGC-803 ceils were divided into 4 groups： nontranfected, transfected with pCMV-HA, transfected with pCMV-GAPDH-HA, transfected with pCMV-Cdx2-HA. Cdx2 gene and its protein expression was detected by quantitative real-time polymerase chain reaction （qRT-PCR） and Western blot techniques respectively. The effects of Cdx2 overexpression on the growth of MGC-803 ceils in vitro was assessed by measuring MrIT, flow cytometry and transmission electron microscopy analysis. Results In MGC-803 ceils transfected with pCMV-Cdx2-HA, ceil proliferation was significantly suppressed, the ceils was uhrastrueturally destroyed, ceil cycle progression was blocked in G0/G1 and apoptosis rate was higher （ P 〈 0. 05 ） in comparison with nontransfected ceils or cells transfected with empty vector. Conclusion It indicated that Cdx2 gene can suppress the growth of gastric carcinoma and may save as a novel therapeutic target.

关 键 词：胃肿瘤 尾型同源盒基因2 肿瘤生长 

分 类 号：R686[医药卫生—骨科学]