重组人血管内皮抑制素对血管内皮细胞的作用  

Effects of recombinanted human angiostatin on vascular endothelial cell

作　　者：伍尚敏[1] 赵亚南[1] 杨力[2] 王璐[2] 郭树忠[2] 刘垠[1] 刘丹[2] 鲁开化[2] 

机构地区：[1]昆明医学院第二附属医院整形外科,云南昆明650101 [2]第四军医大学西京医院全军整形外科研究所,陕西西安710032

出　　处：《中国美容医学》2008年第5期685-688,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨重组人血管内皮抑制素(YH-16)对血管内皮细胞(EC)的作用。方法:传代培养ECV304,将不同浓度的YH-16作用于生长旺盛的EC,以MTT法筛选有效浓度。以有效浓度的YH-16作用于EC,在不同时相点光镜下观察EC形态,MTT检测细胞活性,流式细胞术检测细胞周期,实时荧光定量PCR检测VEGF的表达量。结果:YH-16作用后,光镜下可见EC发生弥散性坏死。MTT检测显示吸光度降低,流式细胞术示G2期细胞比例增多,实时荧光定量PCR检测示VEGF表达量明显降低。结论:YH-16可明显抑制EC的生长增殖,可能通过抑制EC分泌VEGF,从而抑制EC增殖、迁移及血管的形成。Objective To investigate the function of recombinanted human angiostatin （YH-16） on vascular endothelial cell （EC）. Methods （1） Different dosage of YH-16 was used in the vascular endothelial cell, and the effective concentration （10μg/ml） of YH-16 was examined by MTT method.（2）The test was divided into three groups randomly as YH-16 - experiment group, PBS- blank control group and RPMI-1640 medium - positive group.（3）In the following 24h, 48h and 72h interventions, the morphous of EC was observed by photology inverted microscope, the cytoactive was detected by method of MTT and the cell cycle was investigated by flow cytometry （FLC）.The expression of VEGF was dectected by real-time fluorescent quantitate polymerase chain reaction（Real-time PCR）. Results ECs were necrosis in the effect of YH-16, and many cell debris can be seen under the light microscope. The absorptance was degraded by MTT assay （P〈0.05）, and FLC revealed the percentage of G2 stage was mult （P〈0.05）.The express of VEGF was obviously degraded （P〈0.05）. Conclusion YH-16 can suppress the proliferation of vascular endothelial cell actively and it can suppress the DNA synthesis. Simultaneously, it can inhibit the secreting of VEGF, and suppress the prolifetation of EC, also migration and angiogenesis.

关 键 词：重组人血管内皮抑制素 血管内皮细胞 细胞培养 

分 类 号：R96[医药卫生—药理学]