2型糖尿病家系对氧磷酯酶2 C311S基因多态性研究  

Paraoxonase 2 C311S gene polymorphism in pedigree of type 2 diabetes mellitus

作　　者：叶欣[1] 焦凯[1] 张宏柳[1] 侯军峰[1] 张菊[2] 

机构地区：[1]第四军医大学唐都医院内分泌科,陕西西安710038 [2]第四军医大学全军基因诊断研究所

出　　处：《西北国防医学杂志》2008年第3期213-215,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:探讨对氧磷酯酶2(PON2)Cys 311 Ser(C311S)基因多态性与2型糖尿病(T2DM)的关系。方法:应用荧光偏振-模板依赖的染料掺入反应法(TDI-FP)技术,对135例的T2DM家系成员和45例正常人的PON2 C311S基因多态性进行研究。结果:测序结果与TDI-FP方法检测结果比较,符合率100%。2型糖尿病家系中T2DM组、NDR组的基因型频率和等位基因频率与正常对照比较无显著差异(P>0.05)。结论:应用TDI-FP技术能准确敏感地检测PON2 C311S基因多态性。未发现PON2 C311S基因多态性与T2DM的发生存在相关性。Objective： To investigate the association of paraoxonase 2 （PON2） Cys311Ser （C311S） gene polymorphism with type 2 diabetes mellitus （T2DM）. Methods： 135 subjects from pedigrees of type 2 diabetes mellitus and 45 normal controls were screened for the presence of polymorphism by template - directed dye - terminator incorporation with fluorescence polarization （TDI - FP） assay. Results： The distribution of PON2 genotypes and allele frequency in patients with type 2 diabetes from pedigrees, non - diabetic first - degree relatives were similar to those in normal controls （ P 〉 0.05 ）. The result of DNA sequence was in accordance with that measured by TDI -FP. Conclusion： TDI -FP can detect PON2 C311S gene polymorphism correctly and sensitively. Paraoxonase 2 C311S gene polymorphism is not associated with type 2 diabetes mellitus.

关 键 词：2型糖尿病 家系 对氧磷酯酶2 TDI—FP技术 单核苷酸多态性 

分 类 号：R587.1[医药卫生—内分泌]