L-脯-L-亮-甘氨酰胺(PLG)的离子束氚标记及其在鼠脑内的分布  

Ion beam tritium labelling of L-prolyl-L-leucyl-glycinamide and its distribution in rat brain

作　　者：俞斌伟[1] 张年宝[1] 谢炳华[1] 秦锐芳[1] 钟德松[1] 要福增[1] 蔡海鸥[2] 徐德隆[2] 余慧贞[2] 王增[2] 

机构地区：[1]中国科学院上海原子核研究所 [2]上海第二医科大学附属瑞金医院

出　　处：《核技术》1990年第11期689-692,共4页Nuclear Techniques

摘　　要：本文用离子束氚标记技术制得氚标记PLG。经磋胶纸层析法纯化,氚化三肽的比活度为6.81GBq/mmol,放化纯度>95％,具有完全的生物活性。由腹腔注射~3H-PLG,直接测定大鼠脑内部分组织的放射性分布,提示用药4.5h后,垂体、纹状体有较高的放射性,而下丘脑和中脑的放射性较低。Tritium labelled L-prolyl-L-leucyl-glycinamide(3H-PLG) was prepared by ion beam method and purified by thin-layer chromatogrphy. After removal of all tritum the tritia-tcd tripeptide was obtained with a specific radioactivity of 0.81-1.92GBq/mmol. The radiochemical purity was over 95% and biological activity remained unchanged. The distribution of radioactivity after intraperitoneal injection of 3H-PLG has been studied by direct measurement of radioactivity in different parts brain. The uptake of radioactivity was high in pituitary and striatum and was low in hypothalamus and midbrain.

关 键 词：氚标记 L-脯-亮-PLG 帕金森病 

分 类 号：R817[医药卫生—影像医学与核医学]