膀胱Cajal间质细胞对膀胱逼尿肌细胞兴奋调控的结构和功能特征的初步研究  

Interstitial cells of Cajal regulates excitability of detrusor myocytes: structure evidence

作　　者：丁砺蠡[1] 方强[1] 封建立[1] 宋波[1] 

机构地区：[1]第三军医大学全军泌尿外科研究所,重庆400038

出　　处：《第三军医大学学报》2008年第12期1118-1121,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30700270)~~

摘　　要：目的探讨膀胱Cajal细胞(ICC细胞)在逼尿肌细胞兴奋调控中所起的作用。方法①透射电镜观察ICC细胞和逼尿肌细胞之间的超微结构联系;②以荧光光漂白恢复(FRAP)对相邻的ICC细胞和逼尿肌细胞进行检测,观察ICC细胞和相邻逼尿肌细胞之间的荧光强度的变化。结果①超微结构可见ICC细胞和逼尿肌细胞之间有缝隙连接;②对与单个ICC细胞相邻的逼尿肌细胞进行荧光漂白后,可发现逼尿肌细胞内的荧光强度有显著恢复(P<0.01),同时ICC细胞的荧光强度逐渐减弱,说明ICC细胞与逼尿肌细胞间有信号通讯的通路。结论①ICC细胞与逼尿肌细胞之间存在结构上联系;②ICC细胞可以将兴奋信号传递给周围的逼尿肌细胞。Objective To investigate the role of bladder interstitial ceils of Cajal （ICCs） in regulating the excitability of detrusor myocytes. Methods 1 ） Ultrastructural connection between ICCs and detrusor myocytes was detected by transmission electron microscope（TEM）. 2） Fluorescence redistribution after photobleaching （FRAP） was carried out to detect the intercellular communication between detrusor myocytes and its neighboring ICCs. Results 1 ） Gap junction between the 2 kinds of cells was confirmed by TEM. 2） After target detrusor myocyte was photobleached, fluorescence intensity in detrusor myocytes was recovered gradually. In the meantime, fluorescence intensity in the ICCs neighboring to the target detrusor myocyte was decreased. This indicates a signal transmission from the neighboring ICCs to the target detrusor myocyte. Conclusion Structural connection is seen between ICCs and detrusor myocytes. Excitability signals might be transferred from ICCs to detrusor myocyte.

关 键 词：ICC细胞 荧光漂白(FRAP) 膀胱逼尿肌 

分 类 号：R322.62[医药卫生—人体解剖和组织胚胎学] R329.27[医药卫生—基础医学]