银杏苦内酯B对重症急性胰腺炎大鼠血浆中白介素12和血小板活化因子的影响  

EFFECT OF GINKGOLIDE B ON PLASMA LEVELS OF INTERLEUKIN-12 AND PAF IN SEVERE ACUTE PANCREATITIS IN RATS

作　　者：邸瑶[1] 夏时海[1] 佟长青[1] 

机构地区：[1]武警医学院,天津300162

出　　处：《中国应用生理学杂志》2008年第2期152-155,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助课题(30300465);武警医学院科研基金项目(WY2002-19)

摘　　要：目的:观察重症急性胰腺炎(SAP)大鼠血浆白介素12(IL-12)、血小板活化因子(PAF)的水平变化,探讨银杏苦内酯B(BN52021)对SAP的治疗作用。方法:Wistar大鼠随机分成SAP模型组(SAP)、BN52021治疗组(BN)和阴性对照组(NC)。前两组以5%牛磺胆酸钠逆行注入主胰管制成SAP模型,NC组仅翻动十二指肠并触摸胰腺数次关腹,制模15 min后,SAP组、NC组经股静脉注射生理盐水,BN组以BN52021静注;应用ELISA技术测定血浆IL-12和PAF水平。结果:各组血浆IL-12水平存在明显差异,BN组在1 h时相点较SAP、NC组显著性升高(P<0.01);SAP组在6 h1、2 h时相点较NC组显著性升高(P<0.01,P<0.05)。各组血浆PAF水平存在明显差异,在1 h时相点,SAP组与BN组PAF含量较NC组均显著升高(P<0.01);在6 h、12 h时相点,SAP组PAF含量显著高于NC组、BN组(P<0.05,P<0.01)。结论:SAP大鼠早期血浆IL-12水平降低,PAF水平显著升高;BN52021对SAP大鼠具有一定的治疗作用,使早期血浆IL-12水平升高,PAF水平下降。Aim： To observe the levels of interleukin-12（IL-12） and platelet activating factor（PAF） in severe acute pancreatitis （SAP） in rats and the efficacy of Ginkgolide B （BN52021） in treating SAP. Methods： Wistar rats were randomly divided into 3 groups： model group （SAP）, treatment group （BIN） and negative control group （NC）. SAP was induced by retrograde infusion of 5% sodium taurocholate into the pancreatic duct in Wistar rats. NC rats only receive abdominal incision. In groups of SAP and NC rats received the femoral vein injection of isotonic Na chloride 15 minutes after induction of SAP; in BiN group,rats received BN52021 instead. After operation rats were sacrificed at 1,6 and 12 hour for plasma IL-12 and PAF determined with ELISA. Results： An increase of IL-12 in group BiN was observed VS group SAP or group NC at 1 h stage（p=0.011 ,P〈0.01）. At 6 h or 12 h stage,an increase of IL-12 in group SAP was observed VS group NC（P〈0.01 ,P〈0.05） .The plasma level of PAF in group SAP or group BiN was increased significantly at 1 h time stage VS group NC（P〈0.001） .At 6 h or 12 h stage, a decrease of PAF in group BiN or group NC was observed VS group SAP（P〈0.05,P〈0.01） .Conclusion： It confirmed that the plasma level of cytokine IL-12 in SAP group was decreased significantly in early stage and it witnessed a remarkable increase of cytokine PAF. The plasma level of IL-12 was increased in early stage but PAF was decreased in rats treatmented by BN52021 which inhibited the development of SAP.

关 键 词：胰腺炎 BN52021 白介素12 血小板活化因子 

分 类 号：R392.11[医药卫生—免疫学] R446.11[医药卫生—基础医学]