核因子-kappa B在肢体缺血预处理早期心肌保护中的作用  

MYOCARDIAL PROTECTION BY NON-INVASIVE LIMB PRECONDITIONING: THE ROLE OF NUCLEAR FACTOR KAPPA-B

作　　者：陈晓光[1] 马虹[1] 王俊科[1] 吴滨阳[1] 

机构地区：[1]中国医科大学附属第一医院麻醉科,辽宁沈阳110001

出　　处：《中国应用生理学杂志》2008年第2期166-169,共4页Chinese Journal of Applied Physiology

基　　金：辽宁省自然科学基金资助项目(20052115)

摘　　要：目的:探讨非创伤性肢体缺血预处理对缺血/再灌注心肌的作用及核因子kappa B(NF-κB)在诱导远隔器官预处理中的可能机制。方法:Wistar大鼠48只,制备心肌缺血/再灌注模型,随机分3组,缺血/再灌注组(I/R组),非创伤性肢体缺血预处理组(PL组),ProDTC+非创伤性肢体缺血预处理组(PL-D组)。观察各组心肌梗死面积,并应用反转录PCR技术,测定心肌组织NF—κB mRNA。结果:心肌梗死面积PL组较I/R组明显减少,分别为34.5%±7.6%和58.5%±8.5%(P<0.05),而PL-D组与I/R组相比无显著差异。与I/R组比较,PL组和PL-D组NF-κB mRNA表达明显减弱(P<0.05);PL-D组NF-κB mRNA表达较PL组亦明显减弱(P<0.05)。结论:非创伤性肢体缺血预处理对缺血再灌注心肌有早期保护作用,NF-κB在肢体缺血预处理的早期心肌保护中起重要作用。Aim： To investigate a possible role for the transcription factor nuclear factor kappa-B（NF-kB）in preconditioning of the heart to ischemia by remote, early protection. Methods： 48 Wistar rats were randomly divided into three experimental groups. In group I/R, the rats underwent 30 min occlusion of the left anterior descending coronary artery, and 120 min reperfusion. In group PI., the rats underwent four cycles of 5 min occlusion and reperfusion of both hind limbs using a tourniquet before the experiment was continued as in Group I/R. In Group PL-D,we administered NF-kB specific inhibitor,ProDTC 125 mg/kg peritoneally, 15 min before IPC. Infarct size as a percentage of the area at risk was determined by triphenyltetrazolium chloride staining. And another 8 rats in each group were killed and myocardium were stored in liquid nitrogen for the measurement of NF-kB mRNA. Results： The myocardial infarct size （IS） was decreased significantly in Group PI. compared with group I/R,and the IS/AAR was 34.5%± 7.6% and 58.5%±8.5 %, respectively（ P 〈 0.05）. The IS/AAR was 54.4 %±8.9 % in group PL-D, and there was no significant difference compared with group I/R （P^0.05）. The NF-kBmRNA was weaker in PI. group than that in I/R Group,but is stronger than that in PbD group（P〈0.05）. There was almost no expression of NF-kB mRNA in PL-D group.Conclusion： Noninvasive limb IPC is effective in protecting the myocardium from ischemia reperfusion injury. NF-kB plays an important role in the mechanism of this acute remote preconditioning.

关 键 词：肢体缺血预处理 缺血/再灌注损伤 早期保护作用 NF-κB 

分 类 号：R542.2[医药卫生—心血管疾病]