何首乌蒽醌苷类化合物抗肿瘤作用研究  

Study on anti-tumor effects of anthraquinone glycosides from Polygonum multiflorum

作　　者：孙桂波[1] 邓响潮[2] 郭宝江[3] 李续娥[3] 徐惠波[4] 孙晓波[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100094 [2]广州中医药大学,广州510405 [3]华南师范大学生命科学学院,广州510631 [4]吉林省中医药科学院,长春130021

出　　处：《中国新药杂志》2008年第10期837-841,共5页Chinese Journal of New Drugs

基　　金：广东省植物发育工程重点实验室开放基金(200405)

摘　　要：目的:探讨何首乌蒽醌苷类化合物(AGPMT)的抗肿瘤作用及对化疗药物环磷酰胺(CTX)的减毒增效作用,并从免疫学角度初步探讨其作用机制。方法:建立小鼠整体前胃癌(MFC)和肉瘤(S180)移植性肿瘤模型,研究AGPMT的抗肿瘤作用及其对CTX的减毒增效作用,MTT法检测肿瘤细胞的死亡率,XTT法测定T和B淋巴细胞增殖能力;小鼠胸腺细胞增殖法测定白介素-1(IL-1)活性;中性红染色法观察小鼠脾细胞分泌肿瘤坏死因子(TNF)的活性。结果:AGPMT对小鼠MFC实体肿瘤和S180肉瘤均有生长抑制作用,但对体重增长没有影响。AGPMT可以增加CTX对S180荷瘤小鼠的抑瘤作用,同时减轻CTX对S180荷瘤小鼠外周血白细胞数减少的毒性作用。AGPMT能促进S180荷瘤小鼠的T和B淋巴细胞增殖,增加IL-1生成,适度降低TNF。结论:AGPMT具有明显的抗肿瘤作用,对CTX具有减毒增效作用,其抗肿瘤作用可能与提高机体的免疫能力有关。Objective： To study anti-tumor effects of anthraquinone glycosides multiflorum Thunb. （AGPMT） and their effect on reducing toxicity and enhancing from the tuber of Polygonum cyclophosphamide （CTX）. Methods： The above effects of AGPMT were determined by transplant tumor models, evaluated by the inhibitory effect on MFC and S180 tumor growth in mice. The mortality of tumor cell was determined by MTT method, and T and B lymphocyte proliferation was determined by XTT method. IL-1 activity was determined by the mice thymocyte proliferation method. TNF activity in mouse spleen cells was assayed by neutral red assay method. Results： AGPMT had the inhibitory effects on tumor cell growth of mice MFC and sarcoma S180, and reducing toxicity induced by CTX, but had no effect on the body weight in vivo. AGPMT could significantly accelerate T and B lymphocytes proliferation, induce IL-1 production, and reduce TNF content in vitro. Conclusion： AGPMT have significantly anti-tumor effects, and can reduce toxicity induced by CTX. The mechanism of anti-tumor effects of AGPMT might be related to the increased immunity function of mice.

关 键 词：何首乌葸醌苷 肿瘤 免疫 

分 类 号：R285.5[医药卫生—中药学] R286.91[医药卫生—中医学]