芍芪多苷对小鼠体液免疫的调节作用  

The regulating effects of Shaoqiduogan on the humoral immunity in mice

作　　者：王萌[1] 方笋[1] 赵晓娟[1] 陈根林[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药理学安徽省重点实验室,安徽省中药研究与开发重点研究室,抗炎免疫药物安徽省工程技术研究中心,合肥230032

出　　处：《中国新药杂志》2008年第10期842-846,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金资助项目(30572356);安徽省自然科学基金资助项目(050430903);安徽省科技攻关计划项目(06013133B);安徽省高校首批科技创新团队基金(2005TD003);高校博士点科研基金(20060366003);安徽省研究实验基地优秀中青年科研带头人基金(200622805)

摘　　要：目的:探讨在不同免疫状态下,芍芪多苷(SQDG)对小鼠体液免疫的调节作用。方法:采用绵羊红细胞(SRBC)诱导小鼠产生SRBC抗体模型及环磷酰胺(Cy)诱导小鼠免疫增强和抑制模型。小鼠连续灌胃SQDG7d。采用MTT法检测脂多糖(LPS)诱导的小鼠胸腺B淋巴细胞增殖,分光光度法测小鼠血清半数溶血值(HC50)和小鼠脾细胞IgM抗体生成水平。结果:SQDG(120mg·kg-1)灌胃给药7 d能提高正常小鼠的胸腺指数、脾脏指数、血清HC50、脾脏B细胞增殖和脾细胞IgM水平,SQDG(60,120mg·kg-1)对Cy(150mg·kg-1)诱导的免疫低下小鼠的胸腺指数、脾脏指数、血清HC50、脾脏B细胞增殖、脾细胞IgM水平具有明显的恢复作用,SQDG(60,120mg·kg-1)对Cy(250mg·kg-1)诱导的免疫亢进小鼠的胸腺指数、脾脏指数、血清HC50、脾脏B细胞增殖、脾细胞IgM水平均有下调作用。结论:SQDG对小鼠体液免疫反应具有双向调节作用。Objective ：To investigate the regulating effects of Shaoqiduogan （SQDG） on the humoral immu- nity in normal, immunodepressed and immunoenhanced mice. Methods： Production of antibody was induced by sheep red blood cell （SRBC）, and the antibody production was inhibited and potentiated by cyclophosphamide （Cy）. SQDG （30, 60 and 120 mg·kg^-1） were given intragastrically （ig） once daily for 7 consecutive days. LPS-induced proliferation of B thymocytes was measured by MTT assay. The 50% hemolysin value （HC50） was calculated, and formation of IgM antibody in splenic cells was measured with 721 spectrophotometer. Results： In normal immune state, SQDG （120 mg·kg-1 ） increased the indexes of thymus and spleen, HC50 value, IgM level, and LPS-induced B thymocyte proliferation in mice. SQDG （60 and 120 mg·kg^-1） increased and restored all the indexes after Cy （150 mg·kg^-1）-induced immunosuppression, and also reduced and restored all the indexes after Cy （250 mg·kg^-1 ） -induced immunoenhancement. Conclusion： SQDG possesses a dual regulating effect on humoral immunity in mice.

关 键 词：芍芪多苷 体液免疫 胸腺淋巴细胞增殖 溶血素 

分 类 号：R285.5[医药卫生—中药学] R286.95[医药卫生—中医学]