化学发光法快速测定盐酸文拉法辛  

Rapid determination of venlafaxine hydrochloride by chemiluminescence method

作　　者：张庆霞[1] 吴拥军[1] 王伟[1] 

机构地区：[1]郑州大学公共卫生学院

出　　处：《中国新药杂志》2008年第10期866-869,共4页Chinese Journal of New Drugs

基　　金：郑州市科技发展计划项目(052SGY33212)

摘　　要：目的：建立盐酸文拉法辛含量快速检测的化学发光法。方法：Ce^4＋-Na2SO3-H2SO4氧化还原体系能产生微弱的化学发光,在此体系中加入盐酸文拉法辛后发光强度有显著的提高,据此建立了一种测定盐酸文拉法辛的化学发光分析方法。结果：发光信号的增强值与盐酸文拉法辛的质量浓度在1.0×10^-7～8.0×10^-5g·mL^-1的范围内,呈良好的线性关系,平均回收率为100.0%～100.2%。对2.0×10^-6g·mL^-1的盐酸文拉法辛溶液,连续11次平行测定,RSD=1.9%。根据国际纯粹与应用化学联合会（International Union of Pure and Applied Chemistry,简称IUPAC）规定,其检测限为3.8×10^-8g·mL^-1（3σ）,RSD为1.9%。结论：本方法快速、简便、且有较高的灵敏度,可用于胶囊中盐酸文拉法辛的含量测定。Objective：To develop a static chemiluminescence method for the determination of venlafaxine hydrochloride. Methods：Venlafaxine hydrochloride generated the luminous intensity by the weak chemiluminescence reaction in the oxidation-reduction system of Ce^4＋-Na2SO3-H2SO4. Based on this phenomenon,the method was used for the determination of venlafaxine hydrochloride. Results：The linearity of venlafaxine hydrochloride was within 1.0 ×10^-7 ～8.0×10^-5 g·mL^-1. The recovery of venlafaxine hydrochloride was 100.0%～100.4% ,the detection limit was 3.8 ~ 10^-8 g·mL^-1 （3 σ） and the relative standard deviation （ RSD ） of venlafaxine hydrochloride was 1.9%. Conclusion：This easy, rapid and high sensitive method can be used for the quantification of venlafaxine hydrochloride capsules.

关 键 词：盐酸文拉法辛 化学发光 Ce(Ⅳ) 亚硫酸钠 

分 类 号：R927.2[医药卫生—药学] R971.43