EMAP-Ⅱ选择性增加胶质瘤大鼠血肿瘤屏障通透性  

Dose-Response Relation of EMAP-ⅡSelectively Increased the Permeability of Blood-Tumor Barrier in Glioma Rats

作　　者：刘丽波[1] 刘云会[2] 解辉[1] 王萍[1] 薛一雪[1] 

机构地区：[1]中国医科大学神经生物学教研室,辽宁沈阳110001 [2]中国医科大学附属盛京医院神经外科,辽宁沈阳110004

出　　处：《解剖科学进展》2008年第2期113-115,118,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(No.30670723;30570650);辽宁省自然科学基金(No.20052102);沈阳市科学技术计划基金(No.1072033-1-00)

摘　　要：目的研究内皮-单核细胞激活多肽(EMAP-Ⅱ)对脑胶质瘤大鼠血肿瘤屏障(BTB)通透性的影响以及最佳的作用条件。方法应用免疫组织化学方法观察内源性EMAP-Ⅱ在正常脑组织和脑胶质瘤组织中的分布;采用伊文氏兰(EB)法检测不同浓度外源性EMAP-Ⅱ作用后血肿瘤屏障通透性的变化。结果在正常脑组织,内源性EMAP-Ⅱ主要表达在脑微血管和神经元;在脑肿瘤组织,主要表达在肿瘤组织微血管和肿瘤细胞。不同浓度的外源性EMAP-Ⅱ可使血肿瘤屏障对伊文氏兰的通透性增加,对正常脑组织的通透性没有影响,在EMAP-Ⅱ的作用1 h时达到高峰,以后逐渐下降,12 h时基本恢复至灌注前水平;40.g/kg体重的浓度为EMAP-Ⅱ的的最适作用浓度。结论EMAP-Ⅱ能够以剂量依赖的方式选择性增加血肿瘤屏障的通透性。Objective To investigate the effect of endothelial-monocyte activating polypeptide Ⅱ （EMAP-Ⅱ） on the permeability of blood-tumor barrier （BTB） in brain glioma rats and determine the optimal condition of EMAP-Ⅱ. Methods Immunohistochemistry was used to measure the distribution of endogenous EMAP-Ⅱ in the normal brain tissues and the tumor tissues. The permeability of BTB after different concentration of exogenous EMAP-Ⅱ infusion was measured by Evans blue （EB） assay. Results In glioma rats＇ model, immunohistochemistry analysis showed that endogenous EMAP- Ⅱ mainly expressed in the microvessels and neuron in normal brain tissues, while in tumor microvessels and tumor cells in tumor tissues. The effect of different concentration of exogenous EMAP-Ⅱ on BTB permeability for EB extravasation increased, but no normal brain was involved. The permeability of BTB reached a peak at 1 h after administration of exogenous EMAP-Ⅱ, then decreased gradually and restored to the level before administration of exogenous EMAP-Ⅱ. 40 ng/kg tissues is the optimal concentration of exogenous EMAP-Ⅱ. Conclusion EMAP-Ⅱ could increase the permeability of BTB selectively in a dose-dependent manner.

关 键 词：EMAP—Ⅱ 血肿瘤屏障 通透性 

分 类 号：R338.1[医药卫生—人体生理学] R322.81[医药卫生—基础医学]