两种HBV-DNA荧光定量PCR检测比较  

The Comparison of the Two Real-time PCR Fluorescence Detection for Hepatitis B Virus DNA

作　　者：张宪华[1] 

机构地区：[1]河南省安阳市第五人民医院,河南安阳455000

出　　处：《中国现代医生》2008年第15期78-79,共2页China Modern Doctor

摘　　要：目的优化HBV-DNA特异性定量检测方法。方法采用荧光定量PCR检测法,用10份正常血清、10份HBV高滴度(106IU/mL)血清和梯度稀释的HBV克隆质粒标准品,验证两种检测方法的重复性、特异性和灵敏度;并将结果进行配对检验。结果10份正常血清标本均无假阳性HBV-DNA检测结果,特异性极好;10份HBV高滴度血清中检出1份YMDD变异,灵敏度均可达到103IU/mL,重复性和灵敏度两者之间无显著性差异(t=0.702,P>0.05)。结论该方法特异性和灵敏度高,可用于HBV的临床检测和科学研究。Objective To optimize the specific quantitative assay for determining hepatitis B virus DNA （HBV-DNA）. Methods Ten norreal sera samples and ten sera samples with high level（10^6 IU/mL） HBV-DNA, and HBV plasmid standards with 1 ： 10 serial dilutions were used to investigate the specificity, repetition and sensitivity of the two methods by real-time PCR fluorescence detection, then given a statistics analysis tbr the results. Results No significant fluorescent signal was observed when 10 normal sera samples were amplified. One fluorescent signal for YMDD mutation was observed from 10 sera samples with high level HBV-DNA. And lowest hmitation of this assay determined by 1 ： 10 serial dilutions of HBV cloned plasmid was 10^3 IU/mL. There was no significant difference for HBV-DNA results in the two methods （t= 0.702, P〉 0.05 ）. Conclusion Those analyses are highly specific and sensitives, which can be applied in the scientific research of HBV as well as in clinic examination.

关 键 词：乙肝病毒 DNA 实时PCR 荧光定量 

分 类 号：R446[医药卫生—诊断学]