RP-HPLC法测定片剂中盐酸莫索尼定的含量  

RP-HPLC Determination of Moxonidine Hydrochloride Tablets

作　　者：张强[1] 马利敏[1] 张华[1] 肖瑜 汲萍 魏树礼[1] 

机构地区：[1]北京医科大学药学院药剂教研室

出　　处：《药物分析杂志》1997年第6期368-370,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：采用反相高效液相色谱法，选择苯酚为内标物，ＺＹ１１０４型ＸＷＧ－Ｃ１８柱（４．６ｍｍ×２５０ｍｍ）为分析柱，甲醇－水－０．２ｍｏｌ／Ｌ硫酸铵（１０．６８０．７２，ｐＨ＝７．７）为流动相，流速为１．０ｍＬ／ｍｉｎ，检测波长为２５４ｎｍ，进样量２０μＬ，测定片剂中盐酸莫索尼定的含量，按盐酸莫索尼定计算，理论板数为２５４４，样品峰与内标峰的分离度为３．９．线性范围为４～１００ｍｇ／Ｌ（ｒ＝０．９９９８），最低检测量为１０ｎｇ，平均回收率为９９．８２％，ＲＳＤ＝０．７９％．用该法测定３批盐酸莫索尼定片剂的含量，结果分别为（９６．８±１．９）％、（９９．４±１．７）％和（９９．３±１．６）％．本法快速、灵敏、准确，专属性高．A reversed-phase high performance liquid chromatographic method was developed for the determination of moxonidine hydrochloride tablets using a model ZY1104 XWG-C 18 column with phenol as the internal standard.20μL of sample solution was injected, eluted with methanol, water and 0 2mol/L (NH 4) 2SO 4(1∶0 68∶0 72,pH=7 7),and detected by UV spectrometry at 254nm. A linear response ranges from 4 to 100 mg/L(r=0 9998),and the detection limit was 10ng. The analytical recovery was 99 8%, while RSD was 0 79%.Three batches of moxonidine hydrochloride tablets were analyzed,and the results were (96 8±1 9)%、(99 4±1 7)% and (99 3±1 6)% of the labelled contents respectively.

关 键 词：盐酸莫索尼定 HPLC 片剂 

分 类 号：R972.4[医药卫生—药品] R927.2[医药卫生—药学]