血中吩噻嗪类药物硅藻土萃取及紫外导数光谱测定法研究  

Study on Solid-phase Extraction with Celite and Determination by UV Derivative Spectrophotometry of Phenothiazines in Blood

作　　者：谭家镒[1] 姜兆林[1] 郎陈中 孟建效 

机构地区：[1]中国刑警学院

出　　处：《药物分析杂志》1997年第6期391-394,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：研究了血中吩噻嗪类药物及其亚砜的硅藻土固相萃取法．除奋乃静亚砜外，其余药物及亚砜在合适的ｐＨ值条件下，用乙醚洗脱均可得到较高的回收率．奋乃静亚砜用二氯甲烷洗脱可得到高的回收率．洗脱液挥干后用稀硫酸溶解可用紫外二阶导数光谱法测定药物及亚砜含量．本文萃取法和测定法简便快速、灵敏度高，可做为法医毒物分析的常规方法．The method of using celite to extract the phenothiazines and their sulphoxides in blood is presented in this paper.The recoveries of phenothiazines and their sulphoxides,except of fentazin sulphoxide,are about 80% at suitable pH using ether as elutant.The high recoveries of fentazin sulphoxide can be obtained by using dichloromethane as the eluent.After dried,the residue was redissolved in dilute H 2SO 4.The contents of phenothiazines and(or)their sulphoxides were determined by UV 2nd derivative spectrophotometry.The operation of extraction and determination is simple and rapid.The sensitivity is high.The detection limites of phenothiazines and their sulphoxides in blood are below 1mg/L.This method can be used as a routine analytical method in forensic medicine.

关 键 词：硅藻土 固相萃取 紫外导数光谱法 吩噻嗪 法医学 

分 类 号：R89[医药卫生—法医学]