猪Musclin基因的组织表达特征及其与生脂基因的相关性分析  

Expression characterization of Musclin gene in porcine tissues and its correlation with lipogenetic genes

作　　者：姜东凤[1] 孙超[1] 彭永佳[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100

出　　处：《动物学报》2008年第3期453-459,共7页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金(No.30471267);教育部新世纪优秀人才计划(No.NCET-06-0865);学校青年学术骨干计划(No.YAAB-05-22)~~

摘　　要：根据人、大鼠、小鼠Musclin基因序列设计引物,从大白猪肌肉组织提取总mRNA,RT-PCR扩增猪Musclin cDNA;利用半定量(Semi Quantitative,SQ)RT-PCR检测猪Musclin mRNA在不同组织、以及不同生长阶段和不同品种猪脂肪与肌肉组织中的表达量,研究不同品种猪皮下脂肪组织Musclin基因与脂肪酸合成酶(FAS)、过氧化物酶体增殖物激活受体γ(PPARγ)、甘油三酯水解酶(TGH)基因mRNA表达量的相关性。结果表明:猪Musclin基因片段与其他物种的同源性达59%以上,该基因在内脏脂肪和皮下脂肪表达丰度最高,脾脏表达量最低(P<0.05);随月龄增长猪肌肉和皮下脂肪组织中Musclin mRNA表达量呈显著下降(P<0.05);瘦肉型猪肌肉和皮下组织Musclin mRNA表达显著高于脂肪型猪(P<0.05);皮下脂肪组织中该基因mRNA表达与FAS、PPARγ分别呈显著负相关和正相关(P<0.05),与TGH相关性不显著(P>0.05)。因此,猪Musclin基因除在肌肉中表达外,在脂肪等其他组织也能表达;该基因的表达与猪生长阶段、品种有关,且与生脂基因FAS、PPARγ密切相关。推测该基因可能在脂代谢中起到一定的作用。primers were designed based on Musclin gene sequences of human, rat and mice. Total RNA were extracted from muscle tissue of large white pig, then cDNA were amplified using Reverse Transcription-Polymerase Chain Reaction （ RT-PCR）. Levels of Musclin mRNA in different tissues, at different ages and in adipose and muscle tissues of different breeds were measured by Semi Quantitative Reverse Transeription-Polymerase Chain Reaction （SQ RT-PCR）. Correlation were studied between mRNA level of Muselin and that of lipogenetic genes including fatty acid synthetase （ FAS）, peroxisome proliferator-activated receptor γ （PPARγ）, triacylglycerol hydrolase （TGH）. The results showed that Muselin share an gene sequence homology of at least 59% with that of others species ; the highest of Musclin expression levels were found in visceral adipose tissues and subcutaneous adipose tissues and the lowest expression in spleen among all other tissues （P 〈 0.05）; the expression of Musclin mRNA in muscle and subcutaneous adipose tissue were decreased significantly during aging （ P 〈 0.05） ; in the same ages, the levels of Musclin mRNA in subcutaneous adipose and muscle of lean-type pig was significantly higher than that of obesity-type pig （P 〈 0.05 ）; and the express of Musclin was negative correlated and positively correlated with FAS and PPARγ respectively （ P 〈 0.05）. However, it had no significant correlation with TGH （ P 〉 0.05）. So, we concluded that pig Musclin mRNA was expressed in the muscle as well as other tissues such as adipose tissue. The express of porcine Musclin gene was related to age, breed and FAS, PPARγ. it may plays an important role in lipid metabolism .

关 键 词：猪Musclin组织表达FAS PPARΓ TGH 

分 类 号：Q953[生物学—动物学]