中国HIV感染长期不进展者CD4^＋CD25^＋Foxp3^＋调节性T细胞变化研究  

Correlation between CD4^＋CD25^＋Foxp3^＋ regulatory T cells and disease progression in HIV infected long term non-progressors of China

作　　者：张子宁[1] 姜拥军[1] 张旻[1] 刘静[1] 施万英[1] 金鑫[1] 孙国权[1] 王亚男[1] 韩晓旭[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中华微生物学和免疫学杂志》2008年第5期450-453,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（30600532）;国家重点基础研究发展（973）计划（2006CB504206）;重大基础研究前期研究专项（2004CCA02000）

摘　　要：目的对中国HIV感染长期不进展者（LTNP）CD4^＋CD25^＋Foxp3^＋调节性T细胞水平及其与疾病进展相关性进行研究，探讨CD4^＋CD25^＋Foxp3^＋调节性T细胞在LTNP保护机制中发挥的作用。方法选取74名HIV-1感染者（LTNP、典型进展HIV组、AIDS组）及16名健康对照，应用流式细胞仪胞内染色技术在单细胞水平检测CD4^＋CD25^＋Foxp3^＋调节性T细胞表达水平，分析其与CD4^＋T细胞数量、病毒载量、淋巴细胞活化、凋亡水平的相关性。结果中国HIV感染LTNP CD4^＋CD25^＋Foxp3^＋T细胞百分率明显低于典型进展HIV、AIDS组及健康对照组（P〈0．05）。HIV／AIDS患者CD4^＋CD25^＋Foxp3^＋T细胞百分率与CD4^＋T细胞显著负相关（r=-0．509，P〈0．001），与病毒载量明显正相关（r=0．414，P〈0．01），与CD4、CD8^＋T细胞表面CD38、CB95表达水平明显正相关（P〈0．05），与CD4、CD8^＋T细胞表面HIA．DR表达无显著相关性。结论中国HIV感染LTNP CD4^＋CD25^＋Foxp3^＋调节性T细胞百分率明显低于典型进展者，提示调节性T细胞与LTNP保护机制相关。Objective To study the association of CD4^＋CD25^＋Foxp3^＋ regulatory T cells with the HIV long term non-progressors（LTNP） in China. Methods Seventy-four HIV-1 infected patients （ LTNP group, HIV group and AIDS group）and 16 normal controls were enrolled and the frequency of CD4^＋CD25^＋Foxp3^＋ regulatory T cells were detected by flow cytometry. To study the correlation between CD4^＋CD25^＋Foxp3^＋ regulatory T cells and disease progression, the absolute CD4^＋ T cells, viral load, apoptosis and activation of T cells were also examined. Results The frequency of CD4^＋CD25^＋Foxp3^＋ regulatory T cells in LTNP group was significantly lower than that in HIV and AIDS group （ P 〈 0.05 ）. The frequency of CD4^＋CD25^＋Foxp3^＋ regulatory T cells was inversely related to CD4^＋ T cells and closely related to viral load and CD38, CD95 expression on CD4, CD8^＋T cells （ P 〈 0.05 ）. Conclusion The frequency of CD4^＋CD25^＋Foxp3^＋ regulatory T cells of HIV infected LTNP is significantly lower than typical progressors, which indicates that alternation of regulatory T cells may play a protective role in LTNP.

关 键 词：HIV-1 调节性T细胞 长期不进展者 FOXP3 活化 凋亡 

分 类 号：R512.91[医药卫生—内科学]