桥本甲状腺炎患者杀伤细胞免疫球蛋白样受体基因多态性分析  

Killer cell immunoglobulin-like receptor gene polymorphism in Hashimoto＇s thyroiditis patients

作　　者：张海清[1] 赵家军[1] 赵跃然[2] 高聆[2] 廖琳[1] 管庆波[1] 焦玉莲[2] 

机构地区：[1]山东大学附属省立医院内分泌科,济南250021 [2]山东大学附属省立医院中心实验室,济南250021

出　　处：《中华微生物学和免疫学杂志》2008年第5期454-457,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目（30470815,30371304）

摘　　要：目的探讨杀伤细胞免疫球蛋白样受体（killer cell immunoglobulin-like receptor，KIR）基因多态性与桥本甲状腺炎（HT）的关联性。方法选择100例散发HT患者，260例无血缘关系的正常人作为对照，提取全血基因组DNA，采用序列特异性引物聚合酶链反应（PCR—SSP）的方法，对KIR2DL1～5、KIR3DL1—3、KIR2DS1～5、KIR3DS1及KIR2DP1共15个KIR基因进行检测，其中除KIR2DS5基因外，每个基因均采用2对不同的特异性引物。结果HT病例组中KIR2DL5基因的基因频率较对照组显著降低（0．200vs0．312，RR=0．64，P〈0．01）。结论KIR2DL5基因频率的降低可能与HT的发病相关。Objective To investigate the relationship of the killer cell immunoglobulin-like receptor （KIR） gene polymorphism with Hashimoto＇s thyroiditis（HT）. Methods One hundred HT patients and 260 randomly matched healthy controls were enrolled to detect the KIR genotype, The genomic DNA were extracted, and 15 selected KIR genes, KIR2DL1-5, KIR3DL1-3, KIR2DS1-5, KIR3DS1 and pseudogene KIR2DP1, were determined by a polymerase chain reaction using sequence-specific primers （PCR-SSP）. Results The frequency of KIR2DL5 gene was significantly lower of the patient group than that of the control group （0.200 vs0.312, RR=0.64, P〈0.01）. Conclusion Theremay be an association between pathogenesis of HT and KIR2DI.5 gene.

关 键 词：杀伤细胞免疫球蛋白样受体 基因 甲状腺炎 自身免疫性 

分 类 号：R581.4[医药卫生—内分泌] R392.12[医药卫生—内科学]