柠檬黄的ELISA检测方法的建立  

Sandwich ELISA for Detecting Tartrazine

作　　者：邱凯[1] 马国文[1] 柳增善[2] 任立松[2] 沈庆丰[2] 

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028042 [2]吉林大学和平分校区人兽共患病研究所,吉林长春130062

出　　处：《内蒙古民族大学学报（自然科学版）》2008年第3期315-320,共6页Journal of Inner Mongolia Minzu University：Natural Sciences

摘　　要：利用免疫学基本原理,获取抗柠檬黄的单克隆抗体,以此建立了从食品中检测柠檬黄的间接竞争ELISA.试验表明:OVA-TE最适包被浓度为1μg/mL;酶标抗体最适工作浓度为1:3000;酶标抗体的作用温度和时间分别为37℃,1h;封闭液为1%明胶;底物显色时间为15min;对柠檬黄的最低检出量为26.34ng/mL;抗体的最适稀释倍数为1:12000倍;对该方法进行交叉性试验和重复性试验,结果表明此法是一种特异、灵敏、快速的检测方法,并适合大批量样品检测.According to the basic principle of immunology, the monoclonal antibody （McAb） of theanti - TE TE - McAb） were obtained. An indirect competitive inhibition enzyme- linked immunosorbent assay （ic - ELISA） was established to detect the tartrazine in food. The results showed that the mostsuitable concentration of coating the OVA- TE was lμg/mL; 100btl/well goat anti - mouse IgG- HRP （1：3000） was added and incubated for lh at 37℃ ; 1% gelatin was added to eliminate nonspecific binding by blocking the plastic surface; where protein was not bound. ; the minimum detection of the content of antigen reached 26.34ng/mL; 1 ： 18000 TE - McAb was the best suitable dilution. The method of sandwich ELISA is specific, sensitive, efficient and is suitable for suitable for group detection by the blocking test, cross htest and duplica- tion test.

关 键 词：柠檬黄 间接竞争ELISA法 单克隆抗体 

分 类 号：S81[农业科学—畜牧学]