荧光定量聚合酶链反应对生殖器疱疹病毒感染的检测  

Clinical application of fluorescent quantitative PCR in detection of genital herpesvirus infection

作　　者：陈刚[1] 蒋冬香[1] 王晓[1] 

机构地区：[1]桂林医学院附属医院检验科,广西桂林541001

出　　处：《检验医学与临床》2008年第12期717-718,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨荧光定量聚合酶链反应(FQ-PCR)在诊断生殖器疱疹病毒感染中的应用。方法本研究共分两组,检测组即就诊的疑似生殖器疱疹患者347例,对照组12例。分别用FQ-PCR方法检测两组送检标本中的单纯疱疹病毒(HSV)DNA。结果347例疑似生殖器疱疹患者送检标本中,HSV DNA阳性139例,阳性率为40.1%(139/347),其中阳性标本中有皮损组织液75例、男性尿道拭子31例、女性宫颈分泌物33例,各类标本的阳性率依次为91.5%(75/82)、21.1%(31/147)、28.0%(33/118)。HSV DNA阳性人群中性别差异无统计学意义(P>0.05,χ2检验)。12例对照标本中没有检出HSV DNA。结论分泌物中检出的HSV DNA能直观地反映患者当前病毒感染状况,在有皮损组织的患者中,HSV DNA的检出率更高,FQ-PCR能准确、快速地对生殖器疱疹病毒感染做出诊断。Objective TO explore the clinical application of fluorescence quantitative polymerase chain reaction （FQ-PCR） in detection of genital herpesvirus infection. Methods The study was performed on 347 doubtful genital herpes cases （experimental group） and 12 cases of healthy controls （control group）. FQ-POR was applied to detecting herpes simplex-deoxyribonucleic acid （HSV-DNA） in tissue fluids such as vesicle fluids and genital tract swabs. Results In 347 specimens from patients who were suspected to be infected with HSV,there were 139 cases in which H SV-DNA was detected,and the total positive rate was 40.1% （139/347）. Among the 139 PCR-positive specimens, there were 75 cases of tissue fluids, 31 cases of urethral swab and 33 cases of cervix secretions,and the positive rate was 91.5%（75/82）, 21.1%（31/147）, 28.0%（33/118） respectively. The positive rates were no significantly different Via ;（2 test （P〉0.05） between the males and the females. And HSV-DNA wasn＇t detected in the 12 specimens of negative controls. Conclusion The detected HSV-DNA can directly reflect the current infection status of patients, especially for patients with skin lesion, the detection rate of HSV-DNA is more higher. FQ-PCR may be a very good tool in diagnosis of genital herpesvirus infection correctly and rapidly.

关 键 词：疱疹 生殖器 聚合酶链反应 单纯疱疹病毒 

分 类 号：R751[医药卫生—皮肤病学与性病学]