Effects of berberine on L-and T-type calcium channels in guinea pig ventricular myocytes  

作　　者：徐尚忠[1,2,3] 张翼[1,2,3] 任建英[1,2,3] 周兆年 

机构地区：[1]上海市徐汇区中心医院心血管病研究室 [2]河北医科大学生理学教研室 [3]中国科学院上海生理研究所

出　　处：《中国药理学报》1997年第6期518-518,共1页Acta Pharmacologica Sinica

摘　　要：目的：研究小檗碱（Ｂｅｒ）对心室肌细胞钙通道的影响．方法：全细胞膜片箝技术．结果：Ｂｅｒ（１０，３０μｍｏｌ·Ｌ－１）使豚鼠心室肌细胞Ｌ型钙流由１４００±２４７ｐＡ分别减至９７８±２０４ｐＡ及６１７±２３ｐＡ（ｎ＝５，Ｐ＜００５），抑制效应呈浓度依赖及非频率依赖，其电流－电压曲线的峰值下降．Ｂｅｒ（１０μｍｏｌ·Ｌ－１）使Ｌ型钙流失活曲线的最大半激活电压由－２７８ｍＶ变为－３４２ｍＶ，斜率因子由９２２变为１３０３，对激活曲线无影响．Ｂｅｒ（１０，３０μｍｏｌ·Ｌ－１）使Ｔ型钙流峰值由加药前的１５４±８０ｐＡ降至１０１±７８ｐＡ及４８±４５ｐＡ（ｎ＝８，Ｐ＜００５）．结论：Ｂｅｒ对Ｌ和Ｔ型钙通道均有抑制作用．IM: To study the effects of berberine (Ber) on L ( I Ca,L ) and T type ( I Ca,T ) channels in isolated guinea pig ventricular myocytes. METHODS: Using whole cell patch clamp recording technique. RESULTS: Ber 10, 30 μmol·L -1 inhibited the I Ca, L from 1400±247 pA to 978±204 pA ( n =5 cells of 5 guinea pigs, P <0 05), and to 664±179 pA ( n =5, P <0 01), respectively. The inhibitory effect was concentration dependent and non frequency dependent. The peak value of I Ca,L in the current voltage relationship was decreased. Ber affected the inactivation kinetics of I Ca,L . The half activation potential ( V 0 5 ) was shifted from -27 8 mV to -34 2 mV and the slope factor (κ) was changed from 9 22 into 13 03. Ber did not affect the activation kinetics. Ber 10 and 30 μmol·L -1 also inhibited I Ca,T (from 154±80 pA to 101±78 pA, and to 48±45 pA, n =8 cells of 5 guinea pigs, P <0 05).CONCLUSION: Ber possessed blocking effects on both L and T type calcium channels.

关 键 词：小檗碱 心肌 抗心律失常药 离子通道 钙通道 

分 类 号：R285.5[医药卫生—中药学]