细胞因子对乙肝病毒基因疫苗诱导产生抗体的影响  

Effects of cytokines on production of antibody against HBsAg in mice inoculated by HBV genetic vaccine

作　　者：张彤[1] 张光磊[1] 阮丽荣[1] 张绍祖[1] 

机构地区：[1]河南医科大学微生物学与免疫学教研室

出　　处：《中华微生物学和免疫学杂志》1997年第6期421-424,共4页Chinese Journal of Microbiology and Immunology

基　　金：河南省科委科技攻关项目

摘　　要：将构建的三套乙肝病毒基因疫苗（分别编码Ｓ蛋白、ｐｒｅＳ１＋ｐｒｅＳ２＋Ｓ蛋白及ｐｒｅＳ２＋Ｓ蛋白）注射于Ｃ５７ＢＬ／６小鼠胫前肌内。基因疫苗接种３天后，以同样部位注射ｒｈＩＬ－２、ｒｈＩＬ－４、ｒｈＩＬ－６、ｒｈＩＦＮ－γ。运用ＥＬＩＳＡ检测不同时间小鼠血清中乙肝表面抗原（ＨＢｓＡｇ）及抗－ＨＢｓ。结果显示：注射基因疫苗３天内，小鼠体内即可表达ＨＢｓＡｇ；两周后，血清中可测到抗－ＨＢｓ，两月后抗体水平达到高峰，并保持高水平至少两月以上；肌内注射的几种细胞因子均可提高血清中抗－ＨＢｓ水平，与对照组相比差异有极显著性（Ｐ＜０．０１），其中，ｒｈＩＬ－４、ｒｈＩＬ－６、ｒｈＩＦＮ－γ较ｒｈＩＬ－２作用强。提示，细胞因子可作为基因疫苗的佐剂。该研究为增强基因疫苗免疫效果提供了新途径。Three constructs of HBV genetic vaccines containing S gene,preS1+preS2+S gene and preS2+S gene,respectively,were injected into tibialis anterior muscles of C57BL/6 mice.After 3 days of genetic vaccine inoculation,recombinant cytokines rhIL 2,rhIL 4,rhIL 6 and rhIFN γ were administered at the same sites.ELISA was used to detect the HBsAg and anti HBs in mice sera at various times post gene transfer.The results showed that 3 kinds of plasmids expressed and the HBsAg secreted into serum within 3 days post DNA injection.Anti HBs in sera of mice was tested positive ( P/N >2 1) 2 weeks post plasmids injection,the level of antibody attained the peak 6 weeks later,and kept high titer for at least 2 months.Several cytokines were injected intramuscularly and all could significantly ( P <0 01) promote the production of anti HBs induced by HBV vaccine compared with control group.Among the cytokines, rhIL 4, rhIL 6,rhIFN γ showed more powerful adjuvant effects on HBV genetic vaccine than rhIL 2.The results suggest that cytokines can act as immunological adjuvants for genetic vaccines.The research provides a new way for elevation of immune responses induced by genetic vaccines.

关 键 词：细胞因子 乙肝基因疫苗 抗体 

分 类 号：R512.620.1[医药卫生—内科学]