高效液相色谱法测定人血浆中左布比卡因的浓度  

Determination of levobupivacaine in human plasma by high-performance liquid chromatography

作　　者：张小麟[1] 马民玉[2] 王承海[2] 

机构地区：[1]河南工业大学化学化工学院,河南郑州450052 [2]郑州大学第一附属医院麻醉科,河南郑州450052

出　　处：《中国新药与临床杂志》2008年第5期350-352,共3页Chinese Journal of New Drugs and Clinical Remedies

基　　金：河南省科技攻关资助项目(0624420030)

摘　　要：目的建立高效液相色谱法测定人血浆中左布比卡因的浓度。方法以罗哌卡因为内标,色谱柱为Hypersil C_(18)柱(200 mm×4.6 mm,5μm),流动相采用0.01 mol·L^(-1)磷酸二氢钾-乙腈(87:13,V:V,pH=3.4),流速2.0 mL·min^(-1),检测波长为210 nm,柱温40℃。结果标准曲线方程为Y=0.310 1 X+ 0.008 9,r=0.999 7,左布比卡因的质量浓度在0.012 5～2 mg·L^(-1)范围内呈良好的线性关系,最低检测限为0.01 mg·L^(-1);方法回收率在96%～105%之间;日间、日内精密度RSD均<5%。结论本方法可用于临床上左布比卡因血药浓度的监测和药动学研究。AIM To develop a high performance liquid chromatography （HPLC） method for the determination of levobupivacaine in human plasma. METHODS Using ropivacaine as internal standard, the sample was introduced into a LC-6A HPLC system equipped with the column of Hypersil C18 （200 mm×4.6 mm, 5 μm） . The mobile phase was composed of potassium dihydrogen phosphate （0.01 mol·L^-1） and acetonitrile （87 ： 13, V ： V, pH = 3.4）. The flow rate was 2.0 mL·min^-1, the detective wavelength was 210 nm and the temperature of the column was 40 ℃. RESULTS The standard curve was Y = 0.310 1 X ＋ 0.008 9 （r = 0.999 7）. The linear range was 0.012 5-2 mg·L^-1 and the limit of detection was 0.01 mg·L^-1. The average recovery was 96 %-105 %. The RSD of inter-day and intra-day were all less than 5 %. CONCLUSION The HPLC method is suitable for monitoring levobupivacaine concentration in clinical plasma samples and for pharmacokinetics study.

关 键 词：色谱法 高压液相 左布比卡因 血浆 

分 类 号：R969.1[医药卫生—药理学]