银杏叶提取物通过调节IL-13表达水平抑制哮喘大鼠气道黏液高分泌  

Extract of Ginkgo biloba (EGb) blocks mucus hypersecretion of asthmatic rats by inhibiting IL-13

作　　者：黄静[1] 冯玉麟[2] 沈庆[1] 黄方[1] 陈霞[1] 姚丽[1] 

机构地区：[1]重庆市第三人民医院呼吸内科,重庆400014 [2]四川大学华西医院呼吸内科,成都610041

出　　处：《中药药理与临床》2008年第2期39-40,共2页Pharmacology and Clinics of Chinese Materia Medica

基　　金：重庆市卫生局科研课题(05-2-261)

摘　　要：目的:探讨银杏叶提取物(EGb)对哮喘大鼠气道上皮杯状细胞增生和黏液高分泌的抑制作用,以及对哮喘大鼠血清及肺泡灌洗液中IL-13表达水平的影响。方法:以卵蛋白激发制作大鼠哮喘模型,EGb组腹腔内注入EGb作为干预因素,分别以HE、阿辛兰-PAS染色观察气道上皮的改变,杯状细胞的表达及黏液生成,ELISA法检测血清及支气管肺泡灌洗液(BALF)白细胞介素13(IL-13)浓度。结果:哮喘组较对照组气道上皮杯状细胞明显增多,黏液生成增多,血清及肺泡灌洗液中IL-13表达水平显著增高。EGb组较哮喘组杯状细胞数量显著减少,黏液生成减低,血清及肺泡灌洗液中IL-13表达水平明显降低。结论:EGb可以通过抑制IL-13的表达水平减轻哮喘大鼠气道黏液高分泌。Objective： To observe the effects of the extract of Ginkgo biloba（EGb） on mucus hypersecretion of asthmatic rats and the expression of IL-13 in serum and bonchoalveolar lavage fluid（BALF）, Method： Rats were randomly divided into 3 groups ：normal rats ,asthmatic rats , EGb treated rats. Asthmatic rats were sensitized with ovalbumin（OVA）. The assessment of IL-13 in both serum and BALF was performed, histochemical analysis and Alcian blue- periodic acid Schiff （PAS） staining to observe goblet cell. Result： Alcian blue-PAS staining revealed more mucus and goblet dells in the asthmatic bronchial epithelial compared to control rats . meanwhile EGb obviously suppressed the production of mucus and hyperplasia of global cells of asthmatic rats . The productions of IL-13 both in serum or in BALF in asthmatic rats were increased significantly from the normal rats , EGb treatment could strongly reduce the production of IL-13 in both serum and BALF. Conclusion： EGb significantly suppressed the mucus hypersecretion and global cells hyperplasia in asthmatic rats by inhibiting the production of IL- 13.

关 键 词：银杏叶提取物 哮喘 杯状细胞 黏液 L-13 

分 类 号：R285[医药卫生—中药学]