儿童幽门螺杆菌iceA1、babA2基因分布及其细胞免疫功能研究  

Gene distribution and cellular immune function of iceA1 and babA2 from Helicobacter pylori in children

作　　者：高静云[1] 高春燕[1] 高庆双[1] 

机构地区：[1]河北省唐山市妇幼保健院,河北唐山063000

出　　处：《中国病原生物学杂志》2008年第6期411-413,420,共4页Journal of Pathogen Biology

摘　　要：目的探讨儿童幽门螺杆菌(Helicobacterpylori,Hp)iceA1、babA2基因分布特征及其细胞免疫功能。方法对152例有消化道症状患儿行胃镜检查,并在胃窦部取粘膜作Hp的分离培养,利用聚合酶链反应技术(PCR)测定分离培养出的Hp菌株的iceA1、babA2基因,并采用生物素-链霉亲和素(biotin-sreptavidin,BSA)系统检测患者外周血T细胞亚型,ELISA法检测其细胞因子。结果152例中,检出Hp菌株98例,其中iceA1阳性菌株65例,轻度胃炎、中至重度胃炎iceA1阳性率分别为43.59%(17/39)、79.25%(42/53),差异有统计学意义(P<0.01);babA2阳性菌株71例,轻度胃炎、中重度胃炎babA2阳性率分别为48.72%(19/39)、86.79%(46/53),差异有统计学意义(P<0.05);6例活动性胃炎均为iceA1、babA2阳性;iceA1+、babA2+患儿的CD3+、CD4+、CD4+/CD8+和IL-2下降,IL-6、IL-8水平升高(P均<0.001)。结论儿童Hp感染多为iceA1+、babA2+菌株。iceA1+、babA2+Hp菌株为高毒力菌株,易引起较严重的慢性胃炎,使患者细胞免疫功能下降,引起以Th1细胞为主的炎症浸润反应。Objective To investigate the distribution of iceA1 and babA2 gene and to detect cell immunity function in child patients infected with Helicobacter pylori （Hp）. Methods Gastroscopy was performed in 152 children with gastrointestinal symptoms, and gastric biopsy specimens were obtained from antrum and used for isolation of Hp. The types of iceA1 and babA2 from Hp were determined by polymerase chain reaction （PCR）. The T cell subtype of patients was detected by BSA, and to detect the cytokine with ELISA. Results A total of 98 Hp isolates were obtained from 152 children. Of the 98 Hp strains, 66.33%（65/98） had iceA1 gene and 72.45%（71/98） had babA2 gene. The detection rates of iceA1 and babA2 gene in mild chronic gastritis, moderate and severe gastritis, were 43.59% （17/39） and 79.25 % （42/53）, 48.72%（19/39） and 86.79%（46/53）, respectively. 6 cases of reactive gastritis were all iceA1＋ and babA2＋; Furthermore, The percentage of CD3^＋ , CD4^＋ , CD8^＋ , the ratio of CD4^＋/CD8^＋ , and the level of IL-2, IL-6, IL-8 in Hp - iceA1＋ and babA2＋ patients were （45.67±5.89） %, （21.26±3.45） %, （21.65±5.79）%, （1. 08±0.54）%, （1. 29±0.48） mg/L, （110. 38±4.58） ng/L, （115. 46±5.54） ng/L, Respectively, compared with those in Hp-iceA1^- and babA2^- patients, the percentage of CD3^＋ , CD4^＋ and the ratio of CD4^＋/CD8^＋ and the level of IL-2 decreased, but the level of IL-6, IL-8 increased, and the difference was significant （P〈0. 001）. Conclusion Most of Hp infection in children was iceA1＋, babA2＋ strains, and the Hp strains with iceA1＋ and babA2＋ had high virulence, which may play an important role in leading to severe gastritis in children. The Hp with iceA1 ＋, babA2＋ strains may induce the cell immunity function of patients to decrease, and generate the Th1 type infiltration response.

关 键 词：幽门螺杆菌 iceA1基因 babA2基因 细胞免疫功能 儿童 

分 类 号：R378.99[医药卫生—病原生物学]