孕中期弓形虫感染对孕鼠血清孕酮及雌三醇含量的影响  

The decrease of the progesterone and estriol of pregnant mice infected with Toxoplasma gondii

作　　者：赵晋英[1,2] 侯玉英[1] 李艳伟[2] 刘智深[1] 饶华祥[1] 黄泽智[2] 王秀虎[2] 

机构地区：[1]山西医科大学寄生虫学研究所,寄生虫学教研室,山西太原030001 [2]湖南省邵阳医学高等专科学校检验系,湖南邵阳422001

出　　处：《中国病原生物学杂志》2008年第6期425-427,共3页Journal of Pathogen Biology

基　　金：山西省教育厅开发项目(晋教科函[2006]21号)

摘　　要：目的探讨孕中期弓形虫感染对孕鼠血清孕酮(progesterone)及雌三醇(estriol)含量的影响。方法孕中期BALB/c孕鼠随机分为染虫组和健康对照组,染虫组经腹腔无菌接种纯化的RH强毒株弓形虫速殖子,健康对照组注射等量0.01mol/LPBS。分别于妊娠第10、12、14、16、18d,摘孕鼠眼球取血,经放射免疫法测定血清孕酮和雌三醇含量。结果在孕第10、12、14d,孕鼠血清孕酮和雌三醇含量染虫组与对照组比较差异无统计学意义(P>0.05);在孕第16、18d,两组比较差异有统计学意义(P<0.01)。结论孕中期弓形虫感染可引起孕鼠血清孕酮和雌三醇含量下降,这可能是弓形虫感染致胚胎发育迟缓、胎盘组织炎性病变及滋养层细胞凋亡增加的结果,也可能是胚胎发育迟缓、滋养层细胞凋亡增加的原因。Objective To study the effect of Toxoplasma gondii on the progesterone and estriol of pregnant mice. Methods BALB/c pregnant mice were randomly divided into infected group and control group. Experimental model was created by intraperitoneal injection with tachyzoites of the virulent strain RH of T. gondil, while the mice of control group were treated with 0.01 mol/L PBS. Pregnant mice were killed on the 10^th , 12^th , 14^th , 16^th and 18^th day of pregnancy, respectively. The blood of pregnant mice was collected and the serum was separated. The concentration of serum es triol and progesterone of pregnant mice in different pregnancy period was determined by radioimmunoassay. Results The concentrations of serum estriol and progesterone of pregnant mice in the infection group were not different from that of the control group on the 10^th , 12^th , 14^th day of pregnancy （P〉0. 05）, but significantly decreased on the 16^th , 18^th day of pregnancy （P〈0.01）. Conclusion Infection with T. gondii during pregnancy induced the decrease of the concentrations of serum estriol and progesterone of pregnant mice, which may interact with fetus growth retardation and placental inflammatory reaction and enhanced apoptosis.

关 键 词：刚地弓形虫 孕鼠 孕酮 雌三醇 

分 类 号：R382.5[医药卫生—医学寄生虫学]