乙型肝炎病毒感染孕妇胎盘细胞因子的表达与胎盘细胞凋亡的研究(英文)  

EXPRESSION OF PLACENTA CELLULAR FACTORS IN PREGNANT WOMEN INFECTED WITH HEPATITIS B VIRUS AND PLACENTAL CELL APOPTOSIS

作　　者：于爱莲[1] 张延玲[1] 乔云波[2] 邓文[1] 刘丹茹[1] 张忠[1] 王玉[1] 景菲[1] 

机构地区：[1]泰山医学院,山东泰安271000 [2]济南市妇幼保健院

出　　处：《中国病原生物学杂志》2008年第5期330-333,337,F0004,共6页Journal of Pathogen Biology

基　　金：山东省教育厅资助项目(No.JOIK15)~~

摘　　要：目的探讨乙型肝炎病毒感染孕妇胎盘中细胞因子的笔表达以及与胎盘细胞凋亡的关系,阐明乙型肝炎病毒感染孕妇致异常妊娠的机理。方法选择30例HBV感染孕妇死胎胎盘作为研究组,正常分娩后的胎盘20例作为对照组,应用免疫组织化学法测定各组胎盘组织中TNF和IL-18的表达,应用TUNEL法检测胎盘细胞凋亡指数。结果在正常胎盘组织中有一定量的TNF和IL-18的表达,但在HBV感染孕妇死胎胎盘组织中TNF和IL-18的阳性细胞数显著高于对照组(P<0.01);研究组胎盘细胞凋亡指数显著高于对照组(P<0.01),合体滋养细胞的凋亡指数高于滋养细胞,在正常对照组两种细胞的凋亡指数差异有显著性(P<0.05),而在研究组两种细胞的凋亡指数差异无统计学意义(P>0.05)。经相关分析发现,胎盘细胞的凋亡指数与TNF、IL-18呈正相关,(A=6.38,B=0.82,r=0.54;A=9.66,B=0.52,r=0.61)。结论HBV感染孕妇死胎胎盘中TNF和IL-18的表达增加,使胎盘细胞凋亡加速,影响胎盘的正常功能,是导致胎儿异常的原因之一。Objective To investigate the expression of placenta cellular factors in pregnant women infected with hepatitis B virus （HBV） and its relationship with placenta cell apoptosis, and to evaluate the mechanism of the virus resulting in abnormal pregnancy. Methods The cases were divided into two groups： HBV infected stillbirth placental tissue from 30 patients and the normal placental tissue from 20 subjects. Immunohistochemstry was employed to examine the levels of TNF and IL-18 in the tissue and TUNEL assay to determine the placental cell apoptotic index. Results There was a low level of expression of TNF and IL-18 in normal placenta, however the levels of their expression in HBV infected placental tissue were significantly higher than in the control （P〈0.01）. Meanwhile, placental apoptotic index in HBV infected group was higher than in the control （P〈0.01）. Its index in syncytiotrophoblast was greater than in trophoblast. Furthermore, there was a significant difference in apoptotic index between both cells in normal control （P〈0.05）, but no difference in the HBV infected group （P〉0.05）. There was a positive correlation between apoptotic index and expression of TNF as well as IL-18 （A=6.38, B=0.82, r=0.54; A=9.66, B=0.52, r=0.61）. Conclusion In HBV infected stillbirth placenta levels of TNF and IL-18 increase, resulting in a rise in cell apoptosis and impacting placental functions which may lead to the abnormal fetus.

关 键 词：乙型肝炎病毒 胎盘 细胞因子 细胞凋亡 

分 类 号：R373.21[医药卫生—病原生物学]