RP-HPLC法测定丹参缓释片中丹参素含量  

Quantitation Determination of Danshensu in Sustained Release Tables of Salvia Miltiorrhiza by RP HPLC

作　　者：杨瑞芬[1] 李素娟[1] 樊文娟[1] 彭家钢[1] 林宁[1] 

机构地区：[1]湖北中医学院,湖北武汉430065

出　　处：《湖北中医学院学报》2008年第1期34-36,共3页Journal of Hubei College of Traditional Chinese Medicine

基　　金：湖北省教育厅科学技术研究项目;[D200716005]

摘　　要：目的建立丹参缓释片中丹参素的含量测定方法。方法采用RP-HPLC法,色谱柱为Eurospher-100C18（4.6mm×250mm;5μm）;流动相：甲醇-1%冰醋酸（15∶85）;流速：1.0ml/min;紫外检测波长280nm;柱温：室温。结果丹参素的线性范围为9.055-144.879μg/ml;相关系数为0.9999;加样平均回收率为97.34%（RSD=0.80%）。结论该法操作简单,结果准确,重复性好,可作为该制剂的质量控制方法。Objective： To develop a method for the quantitative determination of danshensu in sustained release tables of salvia miltiorrhiza. Method： By RP HPLC the chromatographic column is Eurospher - 100 C18 （46mm ×250mm, 5μm）, the mobile phase is methanol 1% acetic acid （15： 85）, the flow rate is set 10ml/min; the detection wavelength is at 280nm. Results： The linearity ranges of Danshensu are 9. 055 - 144879μg/ml, the correlation is 0. 9999; the average recovery of adding sample is 97. 34% （ RSD =0. 80% ） . Conclusions： The operation is simple, the result is accurate, the reproducible character is good. It is for the quality control of sustained release tables of salvia miltiorrhiza.

关 键 词：丹参素 提取 反相高效液相色谱法 丹参缓释片 

分 类 号：R283.6[医药卫生—中药学]