柴松胚培养诱导芽形成的初步研究  

A Preliminary Study on Inducing Buds of Pinus tabulaeformis f.shekannesis

作　　者：宋维秀[1] 宋西德[1] 周锋利[1] 陈玉红 

机构地区：[1]西北农林科技大学林学院 [2]共和县林业工作站,青海共和813000

出　　处：《西北林学院学报》2008年第3期114-116,共3页Journal of Northwest Forestry University

基　　金：国家科技部科技成果重点推广项目(2001EC0003222)

摘　　要：为了对柴松组织培养进行系统研究，分析了柴松外植体处理方法和不同6-BA浓度对胚培养诱导等形成的影响，结果表明：适宜于柴松芽诱导的培养基为1／2MS＋6-BA 2．0mg·L^-1；经低温沙藏的柴松成熟种胚生长良好，外植体用0．1％HgCl。最佳消毒时间为5．0-5．5min；外植体的最佳放置方式为水平放置。1/2 MS was used as the basic medium for bud proliferation of Pinus tabulaeforrnis f. shekannesis. The best concentration effective of 6-BA was 2. 0 mg · L^-1. The results of selecting the optimal explants showed that the mature embryo which was preserved at low-temperature in sands is the best explants, the suitable disinfectant time in 0.1% HgCl2 for 5.0-5.5 min, the level location of explants was the best way.

关 键 词：柴松 芽诱导 外植体 培养基 

分 类 号：S791.259.04[农业科学—林木遗传育种]