天女木兰组织培养中有效获得无菌外植体的研究  

Studies on Tissue Culture of Magnolia sieboldii to Get Bacteria-free Explants

作　　者：徐石[1] 陆秀君[1] 李天来[1] 

机构地区：[1]沈阳农业大学林学院,辽宁沈阳110161

出　　处：《西北林学院学报》2008年第3期127-129,共3页Journal of Northwest Forestry University

基　　金：沈阳农业大学博士后基金项目(777-230409)

摘　　要：为探讨天女木兰组织培养中无菌外植体的有效获得,将天女木兰外植体分为种子和其他3种(侧芽、带侧芽的茎段、顶芽)外植体进行研究。天女木兰种子分为3种不同类型消毒,进行材料污染率、萌发率及成苗率的统计。其他3种外植体(侧芽、带侧芽的茎段、顶芽)采用L9(34)正交设计,统计无菌率。结果表明:天女木兰种子消毒时间为4 min的Ⅲ型试材为最适外植体,成苗率可达70%。采用正交设计的3种外植体的最优水平组合为A1B2C3,即外植体为侧芽,消毒剂为0.1%HgCl2,消毒时间为12 min。To get nontoxic explant from Magnolia sieboldii tissue culture effectly, the explant of M. sieboldii was divided into seed and other three explants like sprout, stem of sprout and terminal bud for researching deeply. The article takes 3 kinds of sterilizhing to M. sieboldii to statistics the contamination rate, germination rate and seedling rate. Other explants （sprout,stem of sprout and terminal bud） used L9 （3^4） orthogonal design to mearsure pathogen-free rate. The results showed that the best explant was Ⅲ seed of M. sieboldii, its s（erilizhing times was 4 min and seedling rate was high to 70%. The best compose in 3 types of explant using orthogonal design was A1B2C3- explant was sprout, disinfectant was 0.1%HgCl2, sterilizhing times was 12 min.

关 键 词：天女木兰 外植体 正交设计 

分 类 号：S685.790.353[农业科学—观赏园艺]