毛细管气相色谱法测定几种中草药中啶虫眯的痕量残留  

Determination of Acetamiprid Pesticide Residue in the Chinese Herbs by Capillary Gas Chromatography

作　　者：赵燕燕[1] 王翠玲[2] 白洁[1] 苏芳[2] 韩媛媛[1] 李月秋[1] 

机构地区：[1]河北大学医学实验中心,河北保定071000 [2]河北大学药学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2008年第3期272-275,共4页Journal of Hebei University(Natural Science Edition)

基　　金：河北省科技攻关项目(05276101D-88,42761220);河北省中医药管理局资助项目(05015);河北大学人才引进项目(y2004039);河北大学自然科学基金资助项目(2005408)

摘　　要：建立毛细管气相色谱法测定几种中草药中啶虫眯痕量残留的方法．样品经甲醇超声提取、Florisil硅土层析柱净化后，采用不分流进样方式，DB-1弹性石英毛细管柱（30m×0．25mm×0．25μm）经柱程序升温技术，并用电子捕获检测器检测，该方法的线性范围为6．70×10^-4～1．50mg／L，相关系数为0．9991，检测限为6．0×10^-4mg／L，最低检出量为6．0×10^-10mg，被测样品中均含有不同程度的农药残存．本方法检测灵敏度高，重现性好，样品预处理净化效果好，可用于中草药中啶虫眯残留量的检测．To determine acetamiprid pesticide residue in the Chinese herbs by capillary gas chromatography with electron capture detector（CGC-ECD）, Samples were extracted with methanol solvent by ultrasonic, then cleaned up by a column packed with Florisil, The capillary column was DB-1 （30 m × 0.25 mm × 0.25 μm） ; the column temperature program： initial 180 ℃ （hold 2 min）, rate of 10 ℃/min to 250 ℃. The pesticide residue had good linear correlation within 6. 70 × 10^-4 ～ 1.50 mg/L, the limit of detection was 6. 0 × 10^-4mg/L, and the lowest quality of detection was 6.0 × 10^-10 mg. All of samples were detected to have acetamiprid pesticide residue. The method has low detection limit, good purification effection and repeatability. It can be used to determine the trace pesticide residual of acetamiprid in the Chinese herbs.

关 键 词：毛细管气相色谱 中草药 啶虫眯 痕量残留 

分 类 号：O657.71[理学—分析化学]