中华绒螯蟹卵子发生中卵母细胞表面和滤泡细胞的超微结构  

Ultrastructural Study on the Oocyte Surface During Oogenesis in the Chinese Mitten Crab(Eriocheir sinensis)

作　　者：李文艳[1] 康现江[1] 郭明申[1] 穆淑梅[1] 王燕[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2008年第3期295-299,共5页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(303118);河北大学青年科学基金资助项目(2005Q13)

摘　　要：利用透射电镜技术观察了中华绒螯蟹卵子发生过程中卵母细胞表面和滤泡细胞超微结构的变化.结果显示:从卵原细胞期到卵黄大量合成时期,滤泡细胞逐渐向卵母细胞迁移,细胞膜向外伸出许多突起,胞质中的细胞器逐渐增多,它们与卵黄蛋白物质一同移入卵周隙.到卵黄合成晚期,滤泡细胞逐渐解体.与此同时卵母细胞表面在卵黄发生初期可见微胞饮小泡;卵黄大量发生期出现大量微绒毛;卵黄合成晚期,卵黄膜形成,卵黄膜上出现多条通道,这些结构为卵母细胞吸收来自滤泡细胞的卵黄蛋白物质提供了保障.Structural changes of the oocyte surface and follicular cells during oogenesis in the Chinese mitten crab（Eriocheir sinensis） were examined by electron microscopy in order to provide morphological evidence for active intro-oocytic and extro-oocytic yolk formation,The oogonia and follicular cells were unregularly distributed. With the development of ovary, the follicular cells began to move towards the surface of oocytes and gradually surrounded the oocyte membrane. The membrane of the follicular ceils protruded outward. In the cytoplasm, organelle and vitellogenin increased and moved into perivitelline space gradually. The follicular cells disassembled in the final period. The plasma membrane of the oocytes contained many pinocytotic vesicles, microvillus, pores in egg membrane in different stages, which indicated the active extra-oocytic formation.

关 键 词：中华绒螯蟹 卵子发生 卵母细胞 滤泡细胞 

分 类 号：Q248[生物学—细胞生物学]