大叶相思下胚轴离体培养再生植株的研究  

Plant Regeneration from Hypocotyls of Acacia auriculiformis Cultured in vitro

作　　者：刘娟旭[1,2] 刘玲[1] 王静[1] 余义勋[1] 

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]Institute of Food and Agricultural Sciences, University of Florida,Apopka, FL 32703-8504, U. S. A

出　　处：《林业科学研究》2008年第3期403-406,共4页Forest Research

基　　金：霍英东教育基金项目“反义RNA及RNAi技术延长月季切花自然保鲜期(104031)”;广东省自然科学基金项目“基因工程培育具有芳香品质红掌的研究(05300848)”

摘　　要：The regenerated plantlets of Acacia auriculiformis were obtained by the method of re-differentiation of the callus from the hypocotyls explants.The effects of plant regulator compositions on the induction of callus and the differentiation of adventitious buds in vitro culture were studied.The optimum medium for callus induction was MS medium containing 1.0 or 1.5 mg ·L-1 2,4-D and 0.5 mg ·L-1 KT and 100% the callus induction frequency was obtained.The optimum medium for re-differentiation of callus was MS medium containing 1.5 mg ·L-1 6-BA and 0.2 mg ·L-1 NAA and 84.7% of adventitious shoot regeneration frequency with 5.83 shoots per explants was obtained.In addition,the optimum medium for the adventitious buds induced from the hypocotyls explants without transferring the callus was MS medium containing 2.0 mg ·L-1 6-BA,0.1 mg ·L-1 NAA and 0.2 mg ·L-1 KT.Excised shoots were effectively elongated in MS medium without appending any hormones.Elongated shoots of 3.0 cm rooted when they were transferred to MS medium containing 0.1 mg ·L-1 IAA and 0.2 mg ·L-1 NAA after 30 days and developed into healthy plantlets,which resulted in a rooting rate of 85 %.The results of this study will facilitate the application of genetic transformation methods in A.auriculiformis.The regenerated plantlets of Acacia auriculiformis were obtained by the method of re-differentiation of the callus from the hypocotyls explants. The effects of plant regulator compositions on the induction of callus and the differentiation of adventitious buds in vitro culture were studied. The optimum medium for callus induction was MS medium containing 1.0 or 1.5 mg · L^- 1 2,4-D and 0.5 mg · L^- 1KT and 100% the callus induction frequency was obtained. The optimum medium for re-differentiation of callus was MS medium containing 1.5 mg · L ^-1 6-BA and 0.2 mg · L^-1 NAA and 84.7% of adventitious shoot regeneration frequency with 5.83 shoots per explants was obtained. In addition, the optimum medium for the adventitious buds induced from the hypocotyls explants without transferring the callus was MS medium containing 2.0 mg· L^-1 6-BA, 0.1 mg· L^-1 NAA and 0.2 mg· L^-1 KT. Excised shoots were effectively elongated in MS medium without appending any hormones. Elongated shoots of 3.0 cm rooted when they were transferred to MS medium containing 0.1 mg· L^-1 IAA and 0.2 mg · L^-1 NAA after 30 days and developed into healthy plantlets, which resulted in a rooting rate of 85 %. The results of this study will facilitate the application of genetic transformation methods in A. auriculiformis.

关 键 词：大叶相思 下胚轴 植物生长调节剂 植株再生 

分 类 号：S792.99[农业科学—林木遗传育种]