卵形鲳鲹不同组织同工酶表达的差异  

Expression of five kinds of isozyme in different tissues of Trachinotus ovatus

作　　者：齐旭东[1] 区又君[2] 

机构地区：[1]广东海洋大学,广东湛江524025 [2]中国水产科学研究院南海水产研究所,广东广州510300

出　　处：《南方水产》2008年第3期38-42,共5页South China Fisheries Science

基　　金：国家高技术研究发展计划(863计划)项目(2006AA10A414);广东省科技计划项目(C20817);国家“十一五”科技支撑计划项目(2006BAD09A14)

摘　　要：运用聚丙烯酰胺垂直梯度凝胶电泳法对卵形鲳鲹(Trachinotus ovatus)6组织(肌肉、心脏、肝脏、肾脏、脑、脾脏)的5种同工酶(EST、LDH、MDH、ME和AST)进行了初步研究,并对5种同工酶的同工酶位点及其酶谱表型进行了分析。结果表明,卵形鲳鲹的5种同工酶系统具有不同程度的组织特异性。EST由2个基因位点编码,Est-1在这6种组织中都存在,Est-2只存在于肝脏和肾脏中;LDH由3个基因位点编码,共检测到3条谱带,Ldh-2只存在于肾脏中;MDH由2个基因位点编码,Mdh-1表达为MDH1和MDH2 2条酶带,Mdh-2表达为MDH3 1条酶带,在肝脏中的活性最强;ME由1个基因位点编码,只检测到1条酶带,肝脏的含量丰富;AST由2个基因位点编码,只在肝脏和肾脏中检测到,共检测到3条谱带。Five isozymes （EST, ME, AST, LDH and MDH） from six tissues （muscle, heart, liver, kidney, brain and spleen） of Trachinotus ovatus were preliminarily studied using vertical polyacrylamide gradient gel electrophoresis. The results showed that the tissue specificity of these isozymes is very obvious. The expression of enzyme gene loci and the activity of isozyme varied with different tis sues. The number of EST isozyme bands was 3, which was encoded by 2 gene loci. Est-1 expressed in all 6 tissues, and Est-2 only existed in liver and kidney. The number of LDH isozyme bands was 3, which was encoded by 3 gene loci. Ldh-2 only expressed in kidney. Two divisions of MDH were found from T. ovatus. It was encoded by 2 loci, and the most specific MDH was in liver. The isozyme bands of MDH3 also existed in all 6 tissues. The number of ME isozyme band was 1, which was encoded by 1 loci. AST isozyme which was encoded by two loci was only found in liver and kidney.

关 键 词：卵形鲳鲹 同工酶 组织 表达 

分 类 号：Q55[生物学—生物化学]