XE-2100血液分析仪测定有核红细胞的评估  

Performance evaluation of nucleated red blood cell count using the Sysmex XE-2100 automated hematology analyzer

作　　者：杜海珍[1] 束国防[2] 吴岚岚[1] 芦慧霞[2] 丁家华[1] 

机构地区：[1]东南大学附属中大医院血细胞室,江苏南京210009 [2]东南大学附属中大医院检验科,江苏南京210009

出　　处：《现代医学》2008年第2期112-114,共3页Modern Medical Journal

摘　　要：目的对XE-2100血液分析仪测定有核红细胞的性能进行评估。方法通过测定高、中、低值有核红细胞标本评析其重复性、线性范围、携带污染率和稳定性,并对114份7 d内的新生儿静脉血或脐带血标本作常规测定,评价其对有核红细胞的定性报警、定量测定,并与手工涂片法进行对照。结果XE-2100血液分析仪测定有核红细胞的重复性较好,线性范围较广,携带污染率较小,在3 d内稳定性较好。以手工涂片、染色显微镜油镜复检为金标准,其有核红细胞报警的灵敏度为100%,特异度为76.7%。定量计数时与手工法的相关性良好,R2=0.997 8。结论XE-2100血液分析仪对有核红细胞的测定能应用于临床。Objective To evaluate the performance of Sysmex XE-2100 automated hematology analyzer to count nucleated red blood cell （NRBC）. Methods Precision, linearity, carryover and stability were performed by assaying the high, middle, low NRBC samples. The results of 114 neonatal blood or umbilical cord blood samples measured by the Sysmex XE-2100 were compared with manual. Results The System XE-2100 results showed excellent precision linearity and stability. The specificity for NRBC was 76.7% , and sensitivity was 100%. The correlation was excellent between the System XE-2100 and manual（R^2 = 0. 997 8）. Conclusion The performance of NRBC count using the Sysmex XE-2100 automated hematology analyzer is adequate for clinical demand.

关 键 词：血液分析仪 有核红细胞 手工分类 报警 

分 类 号：R446.6[医药卫生—诊断学]