抗体分离纯化技术的研究进展  

Progress in Technology of Antibody Purification and Separation

作　　者：侯越[1] 罗奋华[1] 吴应积[1] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学与生物技术教育部重点实验室,呼和浩特010021

出　　处：《生物技术通报》2008年第3期60-62,共3页Biotechnology Bulletin

基　　金：内蒙古自治区自然科学基金重大项目(编号200408020402)

摘　　要：制备高特异性、高效价的抗体是实验免疫学技术的基础,抗体质量的高低,将直接影响试验的成败。抗体的制备有两个途径:一是一般通用的方法,以纯化的抗原免疫动物,获得多克隆抗体;二是应用杂交瘤技术制备单克隆抗体。但不论用何种技术制备的抗体都需要进行纯化。重要的是根据抗体的性质和来源选择一个合适的分离纯化方法。对当前的纯化方法进行了一个简要的综述。Preparation of antibodies possessed high specificity and affinity is the base of immunology. The success of experiment depends on the quality of antibodies. Preparing antibodies has two ways：one is commonly current method,to be used to obtain polyclonal antibodies via immunizing animals with purified antigen;the other is preparation of monoclonal antibody by using hybridoma technique. Whatever technology used,the prepared antibodies needs to be purified. It is essential to choose the proper procedure for isolation and purification of an antibody,according to properties and source of the antibody. This review is focused on the current purification methods.

关 键 词：抗体 分离 纯化 

分 类 号：R392[医药卫生—免疫学]