Ras基因家族在二羟环氧苯并(a)芘恶性转化细胞中的表达研究  

Study on expression of Ras family gene in transformed cells induced by BPDE

作　　者：周兰兰[1] 谭爱军[2] 蒋义国[1] 沈月兰[1] 傅娟[1] 

机构地区：[1]广州医学院化学致癌研究所与预防医学系,广州510182 [2]珠海市疾病预防控制中心

出　　处：《卫生研究》2008年第3期259-261,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30571546;No.30771780);教育部留学回国人员科研启动基金资助项目(No.2007-24);广东省高校自然科学重点项目(No.06Z021);广东省自然科学基金资助项目(No.07117550)

摘　　要：目的检测环境致癌物苯并(a)芘代谢物二羟环氧苯并芘(BPDE)对人支气管上皮细胞Ras基因家族表达的影响。方法以正常人支气管上皮细胞为对照组,经BPDE恶性转化人支气管上皮细胞为实验组,分别利用RT-PCR、Western blot和免疫细胞化学方法,检测Ras基因家族在mRNA和蛋白水平表达变化。结果RT-PCR实验表明,BPDE恶性转化细胞H-Ras、K-Ras和N-Ras mRNA表达水平分别是正常细胞的2.237、1.254和3.616;Western blot实验显示,H-Ras、K-Ras和N-Ras蛋白表达量分别是正常细胞的1.273倍、1.547和1.600倍;免疫细胞化学实验表明,恶性转化细胞H、K和N-Ras蛋白表达明显强于正常细胞。结论BPDE诱发人支气管上皮细胞恶性转化存在H-Ras、K-Ras和N-Ras基因过表达。Objectlve To explore the expression of Ras family gene in the malignantly transformed human bronchial epithelial cell line induced by anti-benzo（a）pyrene-trans-7,8-dihydrodiol-9,10-epoxide（BPDE）.Methods The levels of H-Ras, K-Ras and N-Ras mRNA expression in BPDE transformed 16HBE cells（16HBE-T） and untransformed control 16HBE cells（16HBE-N） were examined using RT-PCR. Expression levels of those three proteins in both kinds of cells were analyzed by western blot and immunocytochemical method. Results Compared with 16HBE-N, the levels of H-Ras, K-Ras and N-Ras mRNA expression were significantly increased to 2.237, 1.254 and 3.616 times in 16HBE-T, and the protein levels of those genes were increased to 1.273,1.547 and 1.600 times, respectively. Immunocytochemical method showed expression levels of those proteins in 16HBE-T were significantly higher than those in 16HBE-N. Conclusion HRas, K-Ras and N-Ras are overexpressed in 16HBE-T. The activation of oneogenie Ras may participate in malignant transformation of 16HBE induced by BPDE.

关 键 词：Ras基因家族 二羟环氧苯并芘 人支气管上皮细胞 苯并(A)芘 

分 类 号：Q51[生物学—生物化学] R994.6[医药卫生—毒理学]